The role of seasonality on the inhibitory effect of Brazilian green propolis on the oxidative metabolism of neutrophils

Autor(es)

Simoes-Ambrosio, L. M. C. ^[1] ; Gregorio, L. E. ^[2] ; Sousa, J. P. B. ^[2] ; Figueiredo-Rinhel, A. S. G. ^[1] ; Azzolini, A. E. C. S. ^[1] ; Bastos, J. K. ^[2] ; Lucisano-Valim, Y. M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Fis & Quim, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Lab Farmacognosia, Dept Ciencias Farmaceut, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Fitoterapia; v. 81, n. 8, p. 1102-1108, DEC 2010.
• Citações Web of Science: 31

Resumo

The reactive oxygen species (ROS) produced by neutrophils are involved in the pathogenesis of several diseases, for which the intake of antioxidants could benefit patients either as a prophylactic or therapeutic treatment. Propolis is among the known antioxidants, and its chemical composition may vary under the influence of seasonality, which may interfere in its biological properties. This work evaluates the role of seasonality on the production of some important compounds of propolis samples produced monthly from November 2001 through October 2002 as well as the effect of these samples on the oxidative metabolism of stimulated neutrophils, by using both luminol and lucigenin to produce chemiluminescence (CLlum and CLluc, respectively). The cytotoxicity of the most active extracts to neutrophils was also investigated. The inhibitory effect of the propolis samples varied significantly during the studied period for both assays (3.4 +/- 1.1 to 16.0 +/- 1.1 mu g/mL for CLlum and 6.2 +/- 2.0 to 30.0 +/- 5.0 mu g/mL for CLluc), which was also observed in the quantitative profile of the main analyzed compounds (aromadendrin-4'-methyl ether, artepillin C, and baccharin). This effect started to become more prominent during the fall and, among all the studied extracts, the one obtained in May displayed the highest inhibitory effect on CL production (3.4 +/- 1.1 mu g/mL for alum and 6.2 +/- 2.0 mu g/mL for CLluc). The HPLC qualitative profiles of the extracts of propolis samples were quite similar, but there was a huge variation in terms of quantitative profile. It seems that aromadendrin-4'-methyl ether and baccharin play an essential role in the antioxidant activity, while artepillin C is not very important for this effect. The extracts presenting the highest antioxidant activity were produced in May, June, and August, and they did not display cytotoxicity at 25 mu g/mL; quercetin, used as control, was not toxic to neutrophils at 8.5 mu g/mL (C) 2010 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 01/14219-7 - Estudo fitoquímico, realização de ensaios biológicos, cultivo e influência da sazonalidade no metabolismo secundário de Braccharis dracunculifolia e na produção de própolis verde por Apis mellifera
• Beneficiário: Jairo Kenupp Bastos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 02/11469-5 - Estudo do efeito de extratos e frações de própolis verde e de Baccharis dracunculifolia, sobre o metabolismo oxidativo de neutrófilos: influência da sazonalidade e relação com a constituição química
• Beneficiário: Livia Maria Cordeiro Simões Ambrosio
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 06/59893-0 - Desenvolvimento de metodologia analítica e estudo fitoquímico do extrato hidroalcoólico e da fração volátil das folhas e ramos de Copaifera langsdorffii (Leguminosae: Caesalpinioideae)
• Beneficiário: João Paulo Barreto de Sousa
• Modalidade de apoio: Bolsas no Brasil - Doutorado