Contribution of Tris Buffer on Xylitol Enzymatic Production

Autor(es)

Branco, Ricardo de Freitas ^[1] ; Silva, Silvio S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Grp Microbiol Aplicada & Bioproc, Dept Biotechnol, Escola Engn Lorena, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Applied Biochemistry and Biotechnology; v. 162, n. 6, p. 1558-1563, NOV 2010.
• Citações Web of Science: 2

Resumo

Xylitol enzymatic production can be an alternative to chemical and microbial processes, because of advantages like higher conversion efficiency. However, for an adequate conversion, it is necessary to investigate the effect of many parameters, such as buffer initial concentration, pH, temperature, agitation, etc. In this context, the objective of this work was to evaluate xylitol enzymatic production under different Tris buffer initial concentrations in order to determine the best condition for this parameter to begin the reaction. The best results were obtained when Tris buffer initial concentration was 0.22 M, reaching 0.31 g L(-1) h(-1) xylitol volumetric productivity with 99% xylose-xylitol conversion efficiency. Although the increase in buffer concentration allowed better pH maintenance, it hindered the catalysis. The results demonstrate that this bioreaction is greatly influenced by involved ions concentrations. (AU)

• Processo FAPESP: 05/02866-9 - Implementação da nova proposta tecnológica de produção de xilitol usando via enzimática como uma alternativa aos processos fermentativos tradicionais
• Beneficiário: Silvio Silvério da Silva
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/02840-0 - Obtenção enzimática de xilitol usando xilose redutase da Candida guilliermondii como uma alternativa à via fermentativa de produção
• Beneficiário: Ricardo de Freitas Branco
• Linha de fomento: Bolsas no Brasil - Doutorado Direto