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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Contribution of Tris Buffer on Xylitol Enzymatic Production

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Author(s):
Branco, Ricardo de Freitas [1] ; Silva, Silvio S. [1]
Total Authors: 2
Affiliation:
[1] Univ Sao Paulo, Grp Microbiol Aplicada & Bioproc, Dept Biotechnol, Escola Engn Lorena, Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Applied Biochemistry and Biotechnology; v. 162, n. 6, p. 1558-1563, NOV 2010.
Web of Science Citations: 2
Abstract

Xylitol enzymatic production can be an alternative to chemical and microbial processes, because of advantages like higher conversion efficiency. However, for an adequate conversion, it is necessary to investigate the effect of many parameters, such as buffer initial concentration, pH, temperature, agitation, etc. In this context, the objective of this work was to evaluate xylitol enzymatic production under different Tris buffer initial concentrations in order to determine the best condition for this parameter to begin the reaction. The best results were obtained when Tris buffer initial concentration was 0.22 M, reaching 0.31 g L(-1) h(-1) xylitol volumetric productivity with 99% xylose-xylitol conversion efficiency. Although the increase in buffer concentration allowed better pH maintenance, it hindered the catalysis. The results demonstrate that this bioreaction is greatly influenced by involved ions concentrations. (AU)

FAPESP's process: 05/02840-0 - Enzymatic obtaining of xylitol using Candida guilliermondii xylose reductase as an alternative to fermentative production pathway
Grantee:Ricardo de Freitas Branco
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)