| Texto completo | |
| Autor(es): |
Zanon, R. G.
[1]
;
Cartarozzi, L. P.
[1]
;
Victorio, S. C. S.
[1]
;
Moraes, J. C.
[2]
;
Morari, J.
[2]
;
Velloso, L. A.
[2]
;
Oliveira, A. L. R.
[1]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Campinas, Dept Anat, Lab Nerve Regenerat, Inst Biol, Campinas, SP - Brazil
[2] Univ Estadual Campinas, Lab Expt Gastroenterol, Dept Internal Med, Campinas, SP - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY; v. 36, n. 6, p. 515-534, OCT 2010. |
| Citações Web of Science: | 22 |
| Resumo | |
Aims: Major histocompatibility complex (MHC) class I expression by neurones and glia constitutes an important pathway that regulates synaptic plasticity. The upregulation of MHC class I after treatment with interferon beta (IFN beta) accelerates the response to injury. Therefore the present work studied the regenerative outcome after peripheral nerve lesion and treatment with IFN beta, aiming at increasing MHC class I upregulation in the spinal cord. Methods: C57BL/6J mice were subjected to unilateral sciatic nerve crush and treatment with IFN beta. The lumbar spinal cords were processed for immunohistochemistry, in situ hybridization, Western blotting and RT-PCR, while the sciatic nerves were submitted for immunohistochemistry, morphometry and counting of regenerated axons. Motor function recovery was monitored using the walking track test. Results: Increased MHC class I expression in the motor nucleus of IFN beta-treated animals was detected. In the peripheral nerve, IFN beta-treated animals showed increased S100, GAP-43 and p75NTR labelling coupled with a significantly greater number of regenerated axons. No significant differences were found in neurofilament or laminin labelling. The morphological findings, indicating improvements in the regenerative process after IFN treatment were in line with the motor behaviour test applied to the animals during the recovery process. Conclusions: The present data reinforce the role of MHC class I upregulation in the response to injury, and suggest that IFN treatment may be beneficial to motor recovery after axotomy. (AU) | |
| Processo FAPESP: | 05/03159-4 - Influência do interferon beta sobre a reatividade astroglial e plasticidade sináptica após lesão do nervo ciático |
| Beneficiário: | Alexandre Leite Rodrigues de Oliveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 09/07443-0 - Regeneração nervosa periférica através da técnica de tubulização do nervo isquiático e tratamento com interferon beta |
| Beneficiário: | Luciana Politti Cartarozzi |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 06/06673-3 - Impacto da ausencia do interferon gama na plasticidade sinaptica apos lesao do nervo isquiatico. |
| Beneficiário: | Sheila Cristina da Silva Victorio |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |