Bone Deposition, Bone Resorption, and Osteosarcoma

Autor(es)

Caminada Toledo, Silvia Regina ^{[1, 2]} ; Oliveira, Indhira Dias ^{[1, 2]} ; Okamoto, Oswaldo Keith ^[3] ; Zago, Marco Antonio ^[4] ; de Seixas Alves, Maria Teresa ^{[5, 2]} ; Garcia Filho, Reynaldo Jesus ^{[6, 2]} ; Pacheco Donado Macedo, Carla Renata ^[2] ; Petrilli, Antonio Sergio ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Morphol & Genet, BR-04023062 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Pediat, Pediat Oncol Grp, GRAACC, Genet Lab, BR-04023062 Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Neurol & Neurosurg, BR-04023062 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Ctr Cell Based Therapy, Ribeirao Preto, SP - Brazil

^[5] Univ Fed Sao Paulo, Dept Pathol, BR-04023062 Sao Paulo - Brazil

^[6] Univ Fed Sao Paulo, Dept Orthoped Surg & Traumatol, BR-04023062 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ORTHOPAEDIC RESEARCH; v. 28, n. 9, p. 1142-1148, SEP 2010.
• Citações Web of Science: 15

Resumo

Bone deposition and bone resorption are ongoing dynamic processes, constituting bone remodeling. Some bone tumors, such as osteosarcoma (OS), stimulate focal bone deposition. OS is the most common primary bone tumor in children and young adults. A complex network of genes regulates bone remodeling and alterations in its expression levels can influence the genesis and progression of bone diseases, including OS. We hypothesized that the expression profiles of bone remodeling regulator genes would be correlated with OS biology and clinical features. We used real-time PCR to evaluate the mRNA levels of the tartrate-resistant acid phosphatase (ACP5), colony stimulating factor-1 (CSF1R), bone morphogenetic protein 7 (BMP7), collagen, type XI, alpha 2 (COL11A2), and protein tyrosine phosphatases zeta 1 (PTPRZ1) genes, in 30 OS tumor samples and correlated with clinical and histological data. All genes analyzed, except CSF1R, were differentially expressed when compared with normal bone expression profiles. In our results, OS patients with high levels of COL11A2 mRNA showed worse overall (p = 0.041) and event free survival (p = 0.037). Also, a trend for better overall survival was observed in patients with samples showing higher expression of BMP7 (p =0.067). COL11A2 overexpression and BMP7 underexpression could collaborate to OS tumor growth, through its central role in bone remodeling process. (C) 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:1142-1148, 2010 (AU)

• Processo FAPESP: 07/53869-3 - Investigação de eventos genéticos envolvidos no processo de tumorigênese do osteossarcoma
• Beneficiário: Silvia Regina Caminada de Toledo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 04/12150-8 - Expressao genica e polimorfismo em osteossarcoma.
• Beneficiário: Silvia Regina Caminada de Toledo
• Modalidade de apoio: Auxílio à Pesquisa - Regular