Cytogenetical damage in exfoliated oral mucosa cells in elderly people suffering denture stomatitis

Autor(es)

Matsumoto, Mariza A. ^[1] ; Castanho, Juliane ^[1] ; Kawakami, Roberto Y. ^[1] ; Ribeiro, Daniel A. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Cattolica Sacro Cuore, Dept Dent Clin, USC, Bauru, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Biociencias, UNIFESP, BR-11060001 Santos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: GERODONTOLOGY; v. 27, n. 3, p. 183-188, SEP 2010.
• Citações Web of Science: 2

Resumo

Objectives: The aim of this study was to evaluate comparatively the DNA damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) in exfoliated oral mucosa cells from chronic denture stomatitis patients and healthy controls. Background: Over the course of ageing, individuals may develop many diseases such as denture stomatitis. Material and methods: A total of 23 chronic denture stomatitis patients and 23 controls presenting good oral conditions were included in this study. Individuals had epithelial cells mechanically exfoliated, placed in fixative and placed on clean slides, which were checked for nuclear phenotypes. Results: The results indicated no statistically significant differences (p > 0.05) of micronucleated oral mucosa cells from chronic denture stomatitis patients when compared to healthy controls. Nevertheless, chronic denture stomatitis was able to increase other nuclear alterations closely related to cytotoxicity such as karyorrhexis, pyknosis and karyolysis as depicted by significant differences (p < 0.05) between groups. No interaction was observed between smoking and chronic denture stomatitis. Conclusion: In summary, these data indicated that chronic denture stomatitis was able to induce cytotoxic effects as assessed by a micronucleus test. (AU)

• Processo FAPESP: 07/01228-4 - Carcinogênese bucal quimicamente induzida pela 4-nitroquinolina 1-óxido em ratos: possíveis biomarcadores envolvidos em sua patogênese
• Beneficiário: Daniel Araki Ribeiro
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 07/00345-7 - Danos no DNA promovidos por íons metálicos provenientes de aparelhos ortodônticos fixos: análises in vitro e in vivo
• Beneficiário: Daniel Araki Ribeiro
• Modalidade de apoio: Auxílio à Pesquisa - Regular