| Texto completo | |
| Autor(es): |
Fabi, Joao Paulo
[1]
;
Baratelli Carelli Mendes, Luana Regina
[1]
;
Lajolo, Franco Maria
[1]
;
Oliveira do Nascimento, Joao Roberto
[1]
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, FCF, Dept Alimentos & Nutr Expt, Lab Quim Bioquim & Biol Mol Alimentos, BR-05508900 Sao Paulo - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Plant Science; v. 179, n. 3, p. 225-233, SEP 2010. |
| Citações Web of Science: | 21 |
| Resumo | |
Papaya (Carica papaya L) fruit has a short shelf life due to fast ripening induced by ethylene, but little is known about the genetic control of ripening and attributes of fruit quality. Therefore, we identified ripening-related genes affected by ethylene using cDNA-AFLP (Amplified Fragment Length Polymorphism of cDNA). Transcript profiling of non-induced and ethylene-induced fruit samples was performed, and 71 differentially expressed genes were identified. Among those genes some involved in ethylene biosynthesis, regulation of transcription, and stress responses or plant defence were found (heat shock proteins, polygalacturonase-inhibiting protein, and acyl-CoA oxidases). Several transcription factors were isolated, and except for a 14-3-3 protein, an AP2 domain-containing factor, a salt-tolerant zinc finger protein, and a suppressor of PhyA-105 1, most of them were negatively affected by ethylene, including fragments of transcripts similar to VRN1, and ethylene responsive factors (ERF). With respect to fruit quality, genes related to cell wall structure or metabolism, volatiles or pigment precursors, and vitamin biosynthesis were also found. (C) 2010 Elsevier Ireland Ltd. All rights reserved. (AU) | |
| Processo FAPESP: | 02/12452-9 - Metabolismo de carboidratos durante o amadurecimento de frutos: aplicações da genômica funcional |
| Beneficiário: | Franco Maria Lajolo |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 07/56515-8 - Clonagem, expressão e caracterização molecular das enzimas pectinolíticas envolvidas no amolecimento da polpa durante o amadurecimento do mamão papaia |
| Beneficiário: | Joao Paulo Fabi |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 07/56294-1 - Caracterizacao de sequencias de genes diferencialmente expressos durante o amadurecimento do mamao papaia. |
| Beneficiário: | Luana Regina Baratelli Carelli Mendes |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |