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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Aerobic exercise training improves skeletal muscle function and Ca2+ handling-related protein expression in sympathetic hyperactivity-induced heart failure

Texto completo
Autor(es):
Bueno, Jr., C. R. ; Ferreira, J. C. B. ; Pereira, M. G. ; Bacurau, A. V. N. ; Brum, P. C. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Phys Educ & Sport, Dept Biodinam Movimento Corpo Humano, BR-05508900 Sao Paulo - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Journal of Applied Physiology; v. 109, n. 3, p. 702-709, SEP 2010.
Citações Web of Science: 44
Resumo

Bueno CR Jr, Ferreira JC, Pereira MG, Bacurau AV, Brum PC. Aerobic exercise training improves skeletal muscle function and Ca(2+) handling-related protein expression in sympathetic hyperactivity-induced heart failure. J Appl Physiol 109: 702-709, 2010. First published July 1, 2010; doi: 10.1152/japplphysiol.00281.2010.-The cellular mechanisms of positive effects associated with aerobic exercise training on overall intrinsic skeletal muscle changes in heart failure (HF) remain unclear. We investigated potential Ca(2+) abnormalities in skeletal muscles comprising different fiber compositions and investigated whether aerobic exercise training would improve muscle function in a genetic model of sympathetic hyperactivity-induced HF. A cohort of male 5-mo-old wild-type (WT) and congenic alpha(2A)/alpha(2C) adrenoceptor knockout (ARKO) mice in a C57BL/6J genetic background were randomly assigned into untrained and trained groups. Exercise training consisted of a 8-wk running session of 60 min, 5 days/wk (from 5 to 7 mo of age). After completion of the exercise training protocol, exercise tolerance was determined by graded treadmill exercise test, muscle function test by Rotarod, ambulation and resistance to inclination tests, cardiac function by echocardiography, and Ca(2+) handling-related protein expression by Western blot. alpha(2A)/alpha(2C)ARKO mice displayed decreased ventricular function, exercise intolerance, and muscle weakness paralleled by decreased expression of sarcoplasmic Ca(2+) release-related proteins {[}alpha(1)-, alpha(2)-, and beta(1)-subunits of dihydropyridine receptor (DHPR) and ryanodine receptor (RyR)] and Ca(2+) reuptake-related proteins {[}sarco(endo) plasmic reticulum Ca(2+)-ATPase (SERCA) 1/2 and Na(+)/Ca(2+) exchanger (NCX)] in soleus and plantaris. Aerobic exercise training significantly improved exercise tolerance and muscle function and reestablished the expression of proteins involved in sarcoplasmic Ca(2+) handling toward WT levels. We provide evidence that Ca(2+) handling-related protein expression is decreased in this HF model and that exercise training improves skeletal muscle function associated with changes in the net balance of skeletal muscle Ca(2+) handling proteins. (AU)

Processo FAPESP: 05/59740-7 - Exercício físico e controle autonômico na fisiopatologia cardiovascular
Beneficiário:Carlos Eduardo Negrão
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 08/50777-3 - Contribuição da via de sinalização Akt/mTOR na atrofia muscular desencadeada pela insuficiência cardíaca por excesso de catecolaminas: influência do treinamento físico aeróbico
Beneficiário:Aline Villa Nova Bacurau
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 06/57836-0 - Efeito do treinamento físico na expressão de proteínas que transportam Ca2+ e participam do sistema proteolítico dependente de Ca2+ na musculatura esquelética em modelo experimental de insuficiência cardíaca
Beneficiário:Carlos Roberto Bueno Júnior
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 09/03143-1 - Controle de qualidade de proteína na insuficiência cardíaca: papel das diferentes isoformas de proteína quinase C
Beneficiário:Julio Cesar Batista Ferreira
Linha de fomento: Bolsas no Brasil - Pós-Doutorado