Antioxidant enzymatic defense in salivary glands of streptozotocin-induced diabetic rats: a temporal study

Autor(es)

Ibuki, Flavia Kazue ^[1] ; Simoes, Alyne ^[1] ; Nogueira, Fernando Neves ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ctr Pesquisa Biol Oral, Fac Odontol, Dept Mat Dent, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Cell Biochemistry and Function; v. 28, n. 6, p. 503-508, AUG 2010.
• Citações Web of Science: 11

Resumo

Hyperglycemia induces overproduction of superoxide and it is related to diabetic complications. In this study, we analyzed the antioxidant enzymatic defense and the lipid peroxidation of rat salivary glands in six different periods of diabetic condition. Ninety-six rats were divided into 12 groups: C7/14/21128/45/60 (non-diabetic animals) and D7/14/21/28/45/60 (diabetic animals). Diabetes was induced by streptozotocin and the rats were euthanized after 7, 14, 21, 28, 45, or 60 days. Their parotid (PA) and submandibular (SM) glands were removed soon after the sacrifice and the total protein and malondialdehyde (MDA) concentrations, as well as, the superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) activities were determined. Twenty-one days after the diabetes induction, the SM glands showed an increase in SOD, CAT, and GPx activities, as well as, MDA concentration. Concerning the PA glands, an increase in the CAT activity and MDA content was observed throughout the observation period. The results suggest that diabetes can cause alterations on the salivary glands and that PA and SM glands react differently when exposed to diabetes condition. However, no impairment of antioxidant system was observed in the group whose diabetic condition had been induced 60 days earlier, herein named 60-day group. Copyright (C) 2010 John Wiley \& Sons, Ltd. (AU)

• Processo FAPESP: 05/03780-0 - Atividade de SOD, Catalase, GPx e desenvolvimento de estresse oxidativo em glândulas submandibular e parótida de ratos diabéticos induzido por estreptozotocina. Estudo temporal
• Beneficiário: Fernando Neves Nogueira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/04008-0 - Atividade de SOD, catálise, GPx e desenvolvimento de estresse oxidativo em glândulas submandibular e parótida de ratos diabéticos induzidos por streptozotocina: estudo temporal de curto prazo
• Beneficiário: Flávia Kazue Ibuki
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica