Determination of eight fatty acid ethyl esters in meconium samples by headspace solid-phase microextraction and gas chromatography-mass spectrometry

Autor(es)

Roehsig, Marli ^[1] ; Louzada de Paula, Daniela Mendes ^[1] ; Moura, Sidnei ^[1] ; de Albuquerque Diniz, Edna Maria ^[2] ; Yonamine, Mauricio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Coll Pharmaceut Sci, BR-05508900 Sao Paulo - Brazil

^[2] Univ Hosp Sao Paulo, Dept Pediat, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF SEPARATION SCIENCE; v. 33, n. 14, p. 2115-2122, JUL 2010.
• Citações Web of Science: 17

Resumo

A number of fatty acid ethyl esters (FAEEs) have recently been detected in meconium samples. Several of these FAEEs have been evaluated as possible biomarkers for in utero ethanol exposure. In the present study, a method was optimized and validated for the simultaneous determination of eight FAEEs (ethyl laurate, ethyl myristate, ethyl palmitate, ethyl palmitoleate, ethyl stearate, ethyl oleate, ethyl linoleate and ethyl arachidonate) in meconium samples. FAEEs were extracted by headspace solid-phase microextraction. Analyte detection and quantification were carried out using GC-MS operated in chemical ionization mode. The corresponding D5-ethyl esters were synthesized and used as internal standards. The LOQ and LOD for each analyte were <150 and <100 ng/g, respectively. The method showed good linearity (r(2)>0.98) in the concentration range studied (LOQ -2000 ng/g). The intra- and interday imprecision, given by the RSD of the method, was lower than 15% for all FAEEs studied. The validated method was applied to 63 authentic specimens. FAEEs could be detected in alcohol-exposed newborns ( >600 ng/g cumulative concentration). Interestingly, FAEEs could also be detected in some non-exposed newborns, although the concentrations were much lower than those measured in exposed cases. (AU)

• Processo FAPESP: 07/00465-2 - Análise toxicológica dos bioindicadores do etanol e do tabaco em mecônio de recém-nascidos procedentes do berçário do Hospital Universitário - USP
• Beneficiário: Mauricio Yonamine
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/01420-2 - Desenvolvimento e aplicação de método analítico para determinação de ésteres etílicos de ácidos graxos (bioindicadores do etanol) em amostras de mecônio
• Beneficiário: Marli Roehsig
• Modalidade de apoio: Bolsas no Brasil - Mestrado