Global gene expression reveals a set of new genes involved in the modification of cells during erythroid differentiation

Autor(es)

da Cunha, A. F. ^[1] ; Brugnerotto, A. F. ^[2] ; Duarte, A. S. ^[2] ; Lanaro, C. ^[2] ; Costa, G. G. L. ^[2] ; Saad, S. T. O. ^[2] ; Costa, F. F. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Ctr Ciencias Biol & Saude, Dept Genet & Evolucao, BR-13565905 Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Ctr Hematol & Hemoterapia, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Cell Proliferation; v. 43, n. 3, p. 297-309, JUN 2010.
• Citações Web of Science: 4

Resumo

Objectives: Erythroid differentiation is a dynamic process in which a pluripotent stem cell undergoes a series of developmental changes that commit it to a specific lineage. These alterations involve changes in gene expression profiles. In this study, gene expression profiles during differentiation of human erythroid cells of a normal blood donor were evaluated using SAGE. Materials and methods: Global gene expression was evaluated in cells collected immediately before addition of erythropoietin (0 h) and 192 and 336 h after addition of this hormone. Real-time PCR was used to evaluate activation of differentially expressed genes. Results: The data indicate that global aspects of the transcriptome were similar during differentiation of the majority of the genes and that a relatively small set of genes is probably involved in modification of erythroid cells during differentiation. We have identified 93 differentially expressed genes during erythroid development, and expression of some of these was confirmed by qPCR. Various genes including EYA3, ERH, HES6, TIMELESS and TRIB3 were found to be homologous to those of Drosophila melanogaster and here are described for the first time during erythroid development. An important and unique carboxypeptidase inhibitor described in mammalians, LXN, was also identified. Conclusions: The results of this study amplify previously published data and may contribute to comprehension of erythroid differentiation and identification of new target genes involved in some erythroid concerning diseases. (AU)

• Processo FAPESP: 05/51222-7 - Superexpressão e silenciamento dos genes AHSP e Sara1 em células eritroides, análise por SAGE da expressão gênica em cultura eritroide estimulada por eritropoetina, identificação de genes em pacientes talassêmicos homozigotos para a mutação beta 39 (C-T) com evoluções clínicas distintas através de biblioteca subtrativa e estudo in vivo da mutação -195 do promotor de gama globina
• Beneficiário: Anderson Ferreira da Cunha
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 02/13801-7 - Alterações hereditárias das hemoglobinas: genética molecular, aspectos da evolução clínica e produção de animais transgênicos
• Beneficiário: Fernando Ferreira Costa
• Modalidade de apoio: Auxílio à Pesquisa - Temático