Neospora caninum excreted/secreted antigens trigger CC-chemokine receptor 5-dependent cell migration

Autor(es)

Mineo, Tiago W. P. ^{[1, 2]} ; Oliveira, Carlo J. F. ^{[1, 3]} ; Silva, Deise A. O. ^[2] ; Oliveira, Leandro L. ^[1] ; Abatepaulo, Antonio R. ^[1] ; Ribeiro, Damaso P. ^[2] ; Ferreira, Beatriz R. ^[4] ; Mineo, Jose R. ^[2] ; Silva, Joao S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Biochem & Immunol, Sao Paulo - Brazil

^[2] Univ Fed Uberlandia, Inst Biomed Sci, BR-38400902 Uberlandia, MG - Brazil

^[3] NIAID, Lab Malaria & Vector Res, NIH, Rockville, MD 20852 - USA

^[4] USP, Sch Nursing Ribeirao Preto, Dept Maternal Child Nursing & Publ Hlth, BR-14040902 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal for Parasitology; v. 40, n. 7, p. 797-805, JUN 2010.
• Citações Web of Science: 14

Resumo

Neospora caninum, the causative agent of neosporosis, is an obligate intracellular parasite considered to be a major cause of abortion in cattle throughout the world. Most studies concerning N. caninum have focused on life cycle, seroepidemiology, pathology and vaccination, while data on host-parasite interaction, such as host cell migration, mechanisms of evasion and dissemination of this parasite during the early phase of infection are still poorly understood. Here we show the ability of excreted/secreted antigens from N. caninum (NcESAs) to attract monocytic cells to the site of primary infection in both in vitro and in vivo assays. Molecules from the family of cyclophilins present on the NcESAs were shown to work as chemokine-like proteins and NcESA-induced chemoattraction involved G(i) protein signaling and participation of CC-chemokine receptor 5 (CCR5). Additionally, we demonstrate the ability of NcESAs to enhance the expression of CCR5 on monocytic cells and this increase occurred in parallel with the chemotactic activity of NcESAs by increasing cell migration. These results suggest that during the first days of infection, N. caninum produces molecules capable of inducing monocytic cell migration to the sites of infection, which will consequently enhance initial parasite invasion and proliferation. Altogether, these results help to clarify some key features involved in the process of cell migration and may reveal virulence factors and therapeutic targets to control neosporosis. (C) 2010 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 06/06803-4 - Avaliação do papel de receptores do tipo Toll na resposta imune ao Neospora caninum
• Beneficiário: Tiago Wilson Patriarca Mineo
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado