Autologous transplantation of adult adipose derived stem cells into rabbit urethral wall

Autor(es)

Almeida, Fernando Goncalves ^[1] ; Dantas Nobre, Yuri Tulio ^[1] ; Leite, Katia R. ^[2] ; Bruschini, Homero ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Paulista Sch Med, Dept Urol, Sao Paulo - Brazil

^[2] State Univ Sao Paulo, Sch Med, Dept Urol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL UROGYNECOLOGY JOURNAL; v. 21, n. 6, p. 743-748, JUN 2010.
• Citações Web of Science: 12

Resumo

A study was carried out to evaluate the feasibility of autologous adipose derived stem cells (ADSC) transplantation into female rabbits' urethra walls as an alternative to intrinsic urethral regeneration. Inguinal fat pad of 12 New Zealand adult female rabbits were harvested and processed to obtain stromal vascular fraction (SVF). The SVF were platted to isolate ADSC. Before urethral injection, cells were labeled with DiI marker. The urethra wall was injected with 1 x 10(7) autologous cells or saline (sham). The urethra was harvested at 2, 4, and 8 weeks to identify DiI-labeled cells. At 2 and 4 weeks, the ADSCs create a nodule localized in the urethral sub-mucosa. At 8 weeks, the ADSCs spread and integrated with the urethra wall from the initial injection site. This is the first study to demonstrate a successful autologous ADSCs transplantation. It confirms that ADSCs can survive and integrate within the urethral wall. (AU)

• Processo FAPESP: 06/57479-2 - Aplicação de células-tronco na regeneração do mecanismo esfincteriano uretral e tratamento da incontinência urinária de esforço (IUE)
• Beneficiário: Fernando Gonçalves de Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores