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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Autologous transplantation of adult adipose derived stem cells into rabbit urethral wall

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Author(s):
Almeida, Fernando Goncalves [1] ; Dantas Nobre, Yuri Tulio [1] ; Leite, Katia R. [2] ; Bruschini, Homero [2]
Total Authors: 4
Affiliation:
[1] Univ Fed Sao Paulo, Paulista Sch Med, Dept Urol, Sao Paulo - Brazil
[2] State Univ Sao Paulo, Sch Med, Dept Urol, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: INTERNATIONAL UROGYNECOLOGY JOURNAL; v. 21, n. 6, p. 743-748, JUN 2010.
Web of Science Citations: 12
Abstract

A study was carried out to evaluate the feasibility of autologous adipose derived stem cells (ADSC) transplantation into female rabbits' urethra walls as an alternative to intrinsic urethral regeneration. Inguinal fat pad of 12 New Zealand adult female rabbits were harvested and processed to obtain stromal vascular fraction (SVF). The SVF were platted to isolate ADSC. Before urethral injection, cells were labeled with DiI marker. The urethra wall was injected with 1 x 10(7) autologous cells or saline (sham). The urethra was harvested at 2, 4, and 8 weeks to identify DiI-labeled cells. At 2 and 4 weeks, the ADSCs create a nodule localized in the urethral sub-mucosa. At 8 weeks, the ADSCs spread and integrated with the urethra wall from the initial injection site. This is the first study to demonstrate a successful autologous ADSCs transplantation. It confirms that ADSCs can survive and integrate within the urethral wall. (AU)