Snake Venomic of Crotalus durissus terrificus-Correlation with Pharmacological Activities

Autor(es)

Georgieva, Dessislava ^[1] ; Oehler, Michaela ^[2] ; Seifert, Jana ^[2] ; von Bergen, Martin ^[2] ; Arni, Raghuvir K. ^[3] ; Genov, Nicolay ^[4] ; Betzel, Christian ^[1]

Afiliação do(s) autor(es)

^[1] Univ Hamburg, Lab Struct Biol Infect & Inflammat, Inst Biochem & Mol Biol, DESY, D-22603 Hamburg - Germany

^[2] UFZ Helmholtz Ctr Environm Res, Dept Prote, D-04318 Leipzig - Germany

^[3] IBILCE UNESP, Dept Phys, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[4] Bulgarian Acad Sci, Inst Organ Chem, BU-1113 Sofia - Bulgaria

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PROTEOME RESEARCH; v. 9, n. 5, p. 2302-2316, MAY 2010.
• Citações Web of Science: 37

Resumo

The snake venomic of Crotalus durissus terrificus was analyzed by 2-D and 1-D electrophoresis and subsequent MS/MS and enzymatic assays. The venomic of the South American rattlesnake comprises toxins from seven protein families: phospholipases A(2), serine proteinases, ecto-5'-nucleotidases, metalloproteinases, nerve growth factors, phosphodiesterases, and glutaminyl cyclase. The venom toxin composition correlates with the clinical manifestation of the crotalinae snake bites and explains pathological effects of the venom such as neurotoxicity, systemic myonecrosis, hemostatic disorders, myoglobinuria, and acute renal failure. The vast majority of toxins are potentially involved in neurotoxicity, myotoxicity, and coagulopathy. The predominant venom components are neurotoxic phospholipases A2 and serine proteinases. The venom is a rich source of 5'-nucleotidases (7.8% of the identified toxins) inducing hemostatic disorders. Analysis of the venom protein composition provided a catalogue for secreted toxins. The venomic composition of Crotalus d. terrificus and venom gland transcriptome of the synonymous subspecies Crotalus d. collilineatus show differences in the occurrence of protein families and in the abundance of toxins. Some of the venom components identified by the proteomic analysis were not reported in the transcriptome of the Crotalus d. collilineatus venom gland. Enzymatic activities of the Crotalus d. terrificus venom were determined and correlated with the proteomic composition. (AU)

• Processo FAPESP: 07/54865-1 - Applied venom (proteômica aplicada de venenos)
• Beneficiário: Raghuvir Krishnaswamy Arni
• Modalidade de apoio: Auxílio à Pesquisa - Regular