Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Development of microemulsions to topically deliver 5-aminolevulinic acid in photodynamic therapy

Texto completo
Autor(es):
Pires de Campos Araujo, Luciana Mattoso ; Thomazine, Jose Antonio [1] ; Vianna Lopez, Renata Fonseca [2]
Número total de Autores: 3
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Surg & Anat, Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Pharmaceut Sci, BR-14040903 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS; v. 75, n. 1, p. 48-55, MAY 2010.
Citações Web of Science: 48
Resumo

The aim of this study was to obtain and to characterize microemulsions containing 5-aminolevulinic acid (5-ALA) and to investigate the influence of these systems in drug skin permeation for further topical photodynamic therapy (PDT). 5-ALA was incorporated in water-in-oil (W/O), bicontinuous (Bc), and oil-in-water (O/W) microemulsions obtained by the titration of ethyl oleate and PEG-8 caprylic/capric glycerides:polyglyceryl-6 dioleate (3:1) mixtures with water. Selected systems were characterized by conductivity, viscosity, size of the droplets, and drug release. The stability of the drug in the microemulsions was also assessed. Moreover, the in vitro and in vivo skin permeation of 5-ALA was investigated using diffusion cells and confocal scanning laser microscopy (CSLM), respectively. Despite the fact that the O/W microemulsion decreased the 5-ALA diffusion coefficient and retarded the drug release, it also significantly increased the in vitro drug skin permeation when compared to other 5-ALA carriers. It was observed by CSLM that the red fluorescence of the skin increased homogeneously in the deeper skin layers when the 5-ALA microemulsion was applied in vivo, probably due to the formation of the photoactive protoporphyrin IX. The microemulsion developed carried 5-ALA to the deeper skin layers, increasing the red fluorescence of the skin and indicating the potentiality of the system for topical 5-ALA-PDT. (C) 2010 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 04/09465-7 - Laboratório multiusuário de caracterização de sistemas de liberação micro - e nanodispersos de fármacos
Beneficiário:Maria Vitória Lopes Badra Bentley
Linha de fomento: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
Processo FAPESP: 05/01698-5 - Desenvolvimento e caracterização de microemulsões para aplicação na terapia fotodinâmica tópica com o ácido 5-aminolevulínico (ALA)
Beneficiário:Renata Fonseca Vianna Lopez
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 04/05872-7 - Obtenção e caracterização de microemulsões como sistemas de liberação tópica para um fármaco hidrofílico (ALA) e um lipofílico (hexil-ALA) para a terapia fotodinâmica do câncer de pele: estudos de permeação cutânea in vitro e in vivo
Beneficiário:Luciana Mattoso Pires de Campos Araújo
Linha de fomento: Bolsas no Brasil - Mestrado