Helicobacter pylori detection in gastric biopsies, saliva and dental plaque of Brazilian dyspeptic patients

Autor(es)

Rasmussen, Lucas Trevizani ^[1] ; de Labio, Roger William ^[2] ; Gatti, Luciano Lobo ^[3] ; da Silva, Luiz Carlos ^[4] ; de Queiroz, Valdeir Fagundes ^[5] ; Cardoso Smith, Marilia de Arruda ^[6] ; Marques Payao, Spencer Luiz ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sagrado Coracao, Bauru, SP - Brazil

^[2] Fac Med Marilia, Disciplina Genet, Hemoctr, BR-17519050 Marilia, SP - Brazil

^[3] Fac Integradas Ourinhos, Ourinhos, SP - Brazil

^[4] Fac Med Marilia, Disciplina Anat Patol, BR-17519050 Marilia, SP - Brazil

^[5] Fac Med Marilia, Disciplina Cirurgia Sistema Digestorio, BR-17519050 Marilia, SP - Brazil

^[6] Univ Fed Sao Paulo, Dept Morfol, Escola Paulista Med, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Memórias do Instituto Oswaldo Cruz; v. 105, n. 3, p. 326-330, MAY 2010.
• Citações Web of Science: 24

Resumo

Helicobacter pylori is an important human pathogen that causes chronic gastritis and is associated with the development of peptic ulcer disease and gastric malignancies. The oral cavity has been implicated as a potential H. pylori reservoir and may therefore be involved in the reinfection of the stomach, which can sometimes occur following treatment of an H. pylori infection. The objectives of this paper were (i) to determine the presence of H. pylori in the oral cavity and (ii) to examine the relationship between oral H. pylori and subsequent gastritis. Gastric biopsies, saliva samples and dental plaques were obtained from 78 dyspeptic adults. DNA was extracted and evaluated for the presence of H. pylori using polymerase chain reaction and Southern blotting methods. Persons with gastritis were frequently positive for H. pylori in their stomachs (p < 0.0001) and there was a statistically significant correlation between the presence of H. pylori in gastric biopsies and the oral cavity (p < 0.0001). Our results suggest a relationship between gastric infection and the presence of this bacterium in the oral cavity. Despite this, H. pylori were present in the oral cavity with variable distribution between saliva and dental plaques, suggesting the existence of a reservoir for the species and a potential association with gastric reinfection. (AU)

• Processo FAPESP: 06/60836-1 - Caracterizacao dos marcadores de patogenicidade caga e vaca em amostras de placa dental, saliva e mucosa gastrica de pacientes com gastrite.
• Beneficiário: Spencer Luiz Marques Payão
• Modalidade de apoio: Auxílio à Pesquisa - Regular