Transcriptome analysis of nicotine-exposed cells from the brainstem of neonate spontaneously hypertensive and Wistar Kyoto rats

Autor(es)

Ferrari, M. F. R. ^[1] ; Reis, E. M. ^[2] ; Matsumoto, J. P. P. ^[3] ; Fior-Chadi, D. R. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Neurol, Fac Med, BR-01246903 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-01246903 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, BR-01246903 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PHARMACOGENOMICS JOURNAL; v. 10, n. 2, p. 134-160, APR 2010.
• Citações Web of Science: 2

Resumo

In this study, the effects of nicotine on global gene expression of cultured cells from the brainstem of spontaneously hypertensive rat (SHR) and normotensive Wistar Kyoto (WKY) rats were evaluated using whole-genome oligoarrays. We found that nicotine may act differentially on the gene expression profiles of SHR and WKY. The influence of strain was present in 321 genes that were differentially expressed in SHR as compared with WKY brainstem cells independently of the nicotine treatment. A total of 146 genes had their expression altered in both strains after nicotine exposure. Interaction between nicotine treatment and the strain was observed to affect the expression of 229 genes that participate in cellular pathways related to neurotransmitter secretion, intracellular trafficking and cell communication, and are possibly involved in the phenotypic differentiation between SHR and WKY rats, including hypertension. Further characterization of their function in hypertension development is warranted. The Pharmacogenomics Journal (2010) 10, 134-160; doi:10.1038/tpj.2009.42; published online 15 September 2009 (AU)

• Processo FAPESP: 06/00650-1 - Efeitos da nicotina sobre a regulação gênica de sistemas de neurotransmissão envolvidos no controle cardiovascular e gênese da hipertensão em ratos geneticamente hipertensos
• Beneficiário: Merari de Fátima Ramires Ferrari
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado