Nomenclature and guideline to express the amount of a membrane protein synthesized in animal cells in view of bioprocess optimization and production monitoring

Autor(es)

Augusto, Elisabeth F. P. ^[1] ; Moraes, Angela M. ^[2] ; Piccoli, Rosane A. M. ^[1] ; Barral, Manuel F. ^[3] ; Suazo, Claudio A. T. ^[4] ; Tonso, Aldo ^[5] ; Pereira, Carlos A. ^[6]

Afiliação do(s) autor(es)

^[1] IPT, Lab Biotecnol Ind, BR-05508901 Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Fac Engn Quim, Dept Proc Biotecnol, BR-13083970 Campinas, SP - Brazil

^[3] Fundacao Santo Andre, BR-09060650 Santo Andre, SP - Brazil

^[4] Univ Fed Sao Carlos, Dept Engn Quim, BR-13565905 Sao Carlos, SP - Brazil

^[5] Univ Sao Paulo, Escola Politecn, Dept Engn Quim, BR-05508970 Sao Paulo - Brazil

^[6] Inst Butantan, Lab Imunol Viral, BR-05503900 Sao Paulo - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: BIOLOGICALS; v. 38, n. 1, p. 105-112, JAN 2010.
• Citações Web of Science: 8

Resumo

Studies of a bioprocess optimization and monitoring for protein synthesis in animal cells face a challenge on how to express in quantitative terms the system performance. It is possible to have a panel of calculated variables that fits more or less appropriately the intended goal Each mathematical expression approach translates different quantitative aspects We can basically separate them into two categories. those used for the evaluation of cell physiology in terms of product synthesis, which can be for Noprocess improvement or optimization, and those used for production unit sizing and for bioprocess operation With these perspectives and based on our own data of kinetic S2 cells growth and metabolism, as well as on their synthesis of the transmembrane recombinant rabies virus glycoprotein, here indicated as P, we show and discuss the main characteristics of calculated variables and their recommended use Mainly applied to a bioprocess improvement/optimization and that mainly used for operation definition and to design the production unit, we expect these definitions/recommendations would improve the quality of data produced in this field and lead to more standardized procedures In turn, it would allow a better and easier comprehension of scientific and technological communications for specialized readers (C) 2009 The International Association for Biologicals. Published by Elsevier Ltd All rights reserved (AU)

• Processo FAPESP: 02/09482-3 - Expressão de genes heterólogos em células de dípteros: biologia molecular e engenharia de processos
• Beneficiário: Carlos Augusto Pereira
• Linha de fomento: Auxílio à Pesquisa - Temático