Heat-killed Enterococcus faecalis Alters Nitric Oxide and CXCL12 Production but not CXCL8 and CCL3 Production by Cultured Human Dental Pulp Fibroblasts

Autor(es):Mostrar menos -

Sipert, Carla R. ; Moraes, Ivaldo G. ^[1] ; Bernardinelli, Norberti ^[1] ; Garcia, Roberto B. ^[1] ; Bramante, Clovis M. ^[1] ; Gasparoto, Thais H. ; Figueira, Eduardo A. ; Dionisio, Thiago J. ; Campanelli, Ana P. ^[2] ; Oliveira, Sandra H. P. ^[3] ; Cunha, Fernando Q. ^[4] ; Santos, Carlos F. ^[5]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Discipline Endodont, Bauru Sch Dent, BR-17012901 Bauru, SP - Brazil

^[2] Univ Sao Paulo, Discipline Immunol, Bauru Sch Dent, BR-17012901 Bauru, SP - Brazil

^[3] State Univ Sao Paulo, Discipline Pharmacol, Aracatuba Sch Dent, Aracatuba, SP - Brazil

^[4] Univ Sao Paulo, Discipline Pharmacol, Ribeirao Preto Sch Med, BR-14049 Ribeirao Preto - Brazil

^[5] Univ Sao Paulo, Discipline Pharmacol, Bauru Sch Dent, BR-17012901 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENDODONTICS; v. 36, n. 1, p. 91-94, JAN 2010.
• Citações Web of Science: 13

Resumo

Introduction: Fibroblasts are the most abundant cells in dental pulp. To investigate their capacity to produce the chemokines CCL3, CXCL8, and CXCL12 as well as nitric oxide (NO), we evaluated the production of these mediators in supernatants of cultured human dental pulp fibroblasts (HDPF) stimulated by heat-killed Enterococcus faecalis (HKEF). Methods: Primary cultures of HDPF were stimulated with medium alone or HKEF (1:1, 10:1, or 100:1 bacteria:fibroblast) for 1, 6, and 24 hours. Chemokines and NO were assessed through enzyme-linked immunosorbent assay and Griess reaction, respectively. Statistical analysis was performed by using analysis of variance and Tukey post test. Results: CCL3 was not detected, whereas constitutive CXCL8 was not affected. Production of CXCL12 was increased at 1 and 6 hours, and NO was increased at the concentration of 1:1 bacteria:fibroblast at 24 hours. Viability and proliferation assays did not reveal cell number differences. Conclusions: These findings demonstrate that heat-killed E. faecalis is able to increase production of CXCL12 and NO by HDPF. (J Endod 2010;36:91-94) (AU)

• Processo FAPESP: 05/60167-0 - Expressão de pró-colágeno tipo 1, MIP-1± alfa e SDF-1 alfa por fibroblastos da polpa humana estimulados por ácido lipoteicóico de Streptococcus mutans
• Beneficiário: Carlos Ferreira dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Regular