| Texto completo | |
| Autor(es): |
Azzellini, M. Amelia A.
[1]
;
Abbott, Mariana P.
[1]
;
Machado, Alessandra
[2]
;
Miranda, M. Teresa M.
[2]
;
Garcia, Leone C.
[3]
;
Caramori, Giovanni F.
[3, 1]
;
Goncalves, Marcos B.
[4]
;
Petrilli, Helena M.
[4]
;
Ferreira, Ana M. C.
[1]
Número total de Autores: 9
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Dept Quim Fundamental, BR-05508000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Bioquim, Inst Quim, BR-05508000 Sao Paulo - Brazil
[3] Univ Fed Santa Catarina, Dept Quim, BR-88040900 Florianopolis, SC - Brazil
[4] Univ Sao Paulo, Inst Fis, BR-05314970 Sao Paulo - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of the Brazilian Chemical Society; v. 21, n. 7, p. 1303-1317, 2010. |
| Citações Web of Science: | 13 |
| Resumo | |
Interactions of some diimine copper(II) complexes with bovine serum albumin (BSA) were investigated by spectroscopic techniques in order to compare the stability of the complexes and their capability of causing oxidative damage to the protein. The tri- and tetradentate imine ligands employed in this work contain pyridine, pyrazine or imidazole moieties, which are ubiquitous in biological systems. The relative thermodynamic stabilities of the copper(II) complexes were estimated by circular dichroism (CD) using BSA as the competitive ligand. The apparent stability constants determined for the complexes are very similar to one another and to that of the Cu(BSA) complex itself, for which log K Cu(BSA) = 12.9 has already been described in the literature, indicating that the complexes are quite stable under physiological conditions. Two different copper binding sites were evidenced on BSA by spectroscopic measurements (CD, UV-Vis and EPR), depending on the ligand and on the [CuL]:[protein] stoichiometric ratio. Metal binding to any of the sites gives rise to significant protein oxidative damage, especially in the presence of hydrogen peroxide, indicating an oxidative process based on reactive oxygen species (ROS). A small amidated peptide, Asp-Thr-His-NH2, corresponding to the N-terminal region of BSA was synthesized, and its interaction with all the diimine-copper(II) complexes was also investigated in order to clarify the copper imine complex-albumin interactions. Electronic structure calculations at the density functional theory (DFT) level were made to compare the copper-ligand binding energies for each complex with that of the metal coordinated at the N-terminal site of the protein. (AU) | |
| Processo FAPESP: | 05/60596-8 - Espécies complexas com potencial aplicação em bioinorgânica, catálise, farmacologia e química ambiental: concepção, preparação, caracterização e reatividade |
| Beneficiário: | Ana Maria da Costa Ferreira |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |