Analysis of Full-Length Human Immunodeficiency Virus Type 1 Genome Reveals a Variable Spectrum of Subtypes B and F Recombinants in São Paulo, Brazil

Autor(es)

Sa Filho, Dercy José de ^[1] ; Sanabani, Sabri ; Diaz, Ricardo Sobhie ; Munerato, Patrícia ; Brunstein, Adriana ; Fusuma, Erika ; Sabino, Ester Cerdeira ; Janini, Luiz Mario

Afiliação do(s) autor(es)

^[1] Universidade Federal de São Paulo (UNIFESP). Escola Paulista de Medicina. Departamento de Medicina - Brasil

• Tipo de documento: Artigo Científico
• Fonte: AIDS Research and Human Retroviruses; v. 21, n. 2, p. 145-151, Feb. 2005.
• Área do conhecimento: Ciências da Saúde - Medicina
• Assunto(s): Síndromes de imunodeficiência HIV

Resumo

Recombination is one of the major mechanisms contributing to human immunodeficiency virus type 1 (HIV-1) variability. Analysis of pol gene sequences of 215 HIV-1 samples from São Paulo, Brazil classified 189 sequences as subtype B (87.9%), 8 sequences as subtype F (3.7%), and 18 sequences (8.4%) as B/F recombinants. After the analysis of the pol gene, a subset of six recombinant samples composed of sequences with a related recombinant pol structure was selected for full-length genome analysis to identify a possible circulating recombinant form. According to full-length genome analysis, recombination was higher in gag, protease, reverse transcriptase, integrase, and vif. Identification of many distinct recombinant forms and the absence of an identifiable HIV-1 circulating recombinant form suggest that a high frequency of dual infections between HIV-1 subtypes B and F is occurring in São Paulo, Brazil. (AU)

• Processo FAPESP: 03/03513-7 - Caracterização genotípica e fenotípica de recombinantes do vírus da imunodeficiência humana (HIV-1) em amostras do município de Santos/SP
• Beneficiário: Dercy José de Sá Filho
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 03/05184-0 - Caracterizacao genotipica e fenotipica de recombinantes do virus da imunodeficiencia (hiv-1) em amostras do municipio de santos/sp.
• Beneficiário: Ricardo Sobhie Diaz
• Modalidade de apoio: Auxílio à Pesquisa - Regular