Road-killed wild animals: a preservation problem useful for eco-epidemiological studies of pathogens

Autor(es)

Richini-Pereira, V. B. ^[1] ; Bosco, S. M. G. ^[2] ; Theodoro, R. C. ^[2] ; Barrozo, L. ^[3] ; Bagagli, E. ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP Univ Estadual Paulista, Sch Vet Med & Anim Husb, Dept Vet Hyg & Publ Hlth, Botucatu, SP - Brazil

^[2] Sao Paulo State Univ UNESP Univ Estadual Paulista, Botucatu Biosci Inst, Dept Microbiol & Immunol, Botucatu, SP - Brazil

^[3] Univ Sao Paulo, Sch Philosophy Literature & Human Sci, Dept Geog, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 16, n. 4, p. 607-613, 2010.
• Citações Web of Science: 3

Resumo

Road-killed wild animals have been for years used for surveillance of vectors of zoonotic pathogens and may offer new opportunities for eco-epidemiological studies. In the current study, fungal infection was evaluated by PCR and nested-PCR in tissue samples collected from 19 road-killed wild animals. The necropsies were carried out and samples were collected for DNA extraction. Results, using PCR with a panfungal primer and nested PCR with specific primers, indicated that some animals are naturally infected with Amauroascus aureus, Metarhizium anisopliae, Aspergillus flavus, Aspergillus oryzae, Emmonsia parva, Paracoccidioides brasiliensis or Pichia stipitis. The approach employed herein proved useful for detecting the environmental occurrence of several fungi, as well as determining natural reservoirs in wild animals and facilitating the understanding of host-pathogen relationships. (AU)

• Processo FAPESP: 06/03597-4 - Ecologia e evolução do Paracoccidioides brasiliensis: avaliando a ocorrência de espécies crípticas e detecção molecular em animais silvestres em área hiper endêmica de paracoccidioidomicose
• Beneficiário: Eduardo Bagagli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/56771-9 - Ecologia molecular de fungos patogenicos onygenales em animais silvestres do interior do estado de sao paulo.
• Beneficiário: Virgínia Bodelão Richini Pereira
• Modalidade de apoio: Bolsas no Brasil - Doutorado