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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Multiresidue determination of tetracyclines, sulphonamides and chloramphenicol in bovine milk using HPLC-DAD

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Autor(es):
Vargas Mamani, Monica Cecilia [1] ; Reyes Reyes, Felix Guillermo [2] ; Rath, Susanne [1]
Número total de Autores: 3
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, UNICAMP, Dept Analyt Chem, Inst Chem, BR-13084971 Campinas, SP - Brazil
[2] Univ Estadual Campinas, UNICAMP, Dept Food Sci, Sch Food Engn, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Food Chemistry; v. 117, n. 3, p. 545-552, DEC 1 2009.
Citações Web of Science: 66
Resumo

A high-performance liquid chromatographic method for the simultaneous determination of tetracycline, oxytetracycline, chlortetracycline, sulfamethazine, sulfaquinoxaline. sulfamethoxazole and chloramphenicol in milk has been developed. The determination of these antimicrobials was carried out using HPLC-DAD with a C(18) hybrid column and gradient elution with a mobile phase composed of an aqueous phase of 0.075 mol L(-1) sodium acetate, 0.035 mol L(-1) calcium chloride and 0.025 mol L(-1)sodium EDTA, pH 7 and an organic phase of methanol:acetonitrile, 75:25 v/v. Sample preparation involved protein precipitation followed by solid-phase extraction using a polymeric cartridge. The method was validated and applied for the analysis of pasteurised milk samples commercialised in Brazil. The limits of quantitation for all antimicrobials, with the exception of choramphenicol, were below the maximum residue limit, which indicates that the method is appropriate for the determination of these antimicrobials in milk. (C) 2009 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 04/07312-9 - Residuos de antimicrobianos em alimentos:oxitetraciclina e acido oxolinico em peixes.
Beneficiário:Susanne Rath
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 01/14072-6 - Resíduos de antimicrobianos em alimentos: enrofloxacina em carne de frango
Beneficiário:Susanne Rath
Modalidade de apoio: Auxílio à Pesquisa - Regular