Multiresidue determination of tetracyclines, sulphonamides and chloramphenicol in bovine milk using HPLC-DAD

Autor(es)

Vargas Mamani, Monica Cecilia ^[1] ; Reyes Reyes, Felix Guillermo ^[2] ; Rath, Susanne ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Dept Analyt Chem, Inst Chem, BR-13084971 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Dept Food Sci, Sch Food Engn, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food Chemistry; v. 117, n. 3, p. 545-552, DEC 1 2009.
• Citações Web of Science: 66

Resumo

A high-performance liquid chromatographic method for the simultaneous determination of tetracycline, oxytetracycline, chlortetracycline, sulfamethazine, sulfaquinoxaline. sulfamethoxazole and chloramphenicol in milk has been developed. The determination of these antimicrobials was carried out using HPLC-DAD with a C(18) hybrid column and gradient elution with a mobile phase composed of an aqueous phase of 0.075 mol L(-1) sodium acetate, 0.035 mol L(-1) calcium chloride and 0.025 mol L(-1)sodium EDTA, pH 7 and an organic phase of methanol:acetonitrile, 75:25 v/v. Sample preparation involved protein precipitation followed by solid-phase extraction using a polymeric cartridge. The method was validated and applied for the analysis of pasteurised milk samples commercialised in Brazil. The limits of quantitation for all antimicrobials, with the exception of choramphenicol, were below the maximum residue limit, which indicates that the method is appropriate for the determination of these antimicrobials in milk. (C) 2009 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 04/07312-9 - Residuos de antimicrobianos em alimentos:oxitetraciclina e acido oxolinico em peixes.
• Beneficiário: Susanne Rath
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 01/14072-6 - Resíduos de antimicrobianos em alimentos: enrofloxacina em carne de frango
• Beneficiário: Susanne Rath
• Modalidade de apoio: Auxílio à Pesquisa - Regular