Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Multiresidue determination of tetracyclines, sulphonamides and chloramphenicol in bovine milk using HPLC-DAD

Full text
Author(s):
Vargas Mamani, Monica Cecilia [1] ; Reyes Reyes, Felix Guillermo [2] ; Rath, Susanne [1]
Total Authors: 3
Affiliation:
[1] Univ Estadual Campinas, UNICAMP, Dept Analyt Chem, Inst Chem, BR-13084971 Campinas, SP - Brazil
[2] Univ Estadual Campinas, UNICAMP, Dept Food Sci, Sch Food Engn, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Food Chemistry; v. 117, n. 3, p. 545-552, DEC 1 2009.
Web of Science Citations: 66
Abstract

A high-performance liquid chromatographic method for the simultaneous determination of tetracycline, oxytetracycline, chlortetracycline, sulfamethazine, sulfaquinoxaline. sulfamethoxazole and chloramphenicol in milk has been developed. The determination of these antimicrobials was carried out using HPLC-DAD with a C(18) hybrid column and gradient elution with a mobile phase composed of an aqueous phase of 0.075 mol L(-1) sodium acetate, 0.035 mol L(-1) calcium chloride and 0.025 mol L(-1)sodium EDTA, pH 7 and an organic phase of methanol:acetonitrile, 75:25 v/v. Sample preparation involved protein precipitation followed by solid-phase extraction using a polymeric cartridge. The method was validated and applied for the analysis of pasteurised milk samples commercialised in Brazil. The limits of quantitation for all antimicrobials, with the exception of choramphenicol, were below the maximum residue limit, which indicates that the method is appropriate for the determination of these antimicrobials in milk. (C) 2009 Elsevier Ltd. All rights reserved. (AU)