The effect of low-level laser irradiation (In-Ga-Al-AsP-660 nm) on melanoma in vitro and in vivo

Autor(es)

Frigo, Lucio ^[1] ; Luppi, Juliana S. S. ^[1] ; Favero, Giovani M. ^[2] ; Maria, Durnavei A. ^[3] ; Penna, Socrates C. ^[4] ; Bjordal, Jan M. ^{[5, 6]} ; Bensadoun, Rene J. ^[7] ; Lopes-Martins, Rodrigo A. B. ^[4]

Afiliação do(s) autor(es)

^[1] Univ Cruzeiro Sul, Biol Sci & Hlth Ctr, BR-08060070 Sao Paulo - Brazil

^[2] Univ Estadual Ponta Grossa, Dept Gen Biol, BR-84030900 Ponta Grossa, PR - Brazil

^[3] Butantan Inst, Biochem & Biophys Lab, BR-05599000 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Lab Pharmacol & Phototherapy Inflammat, Dept Pharmacol, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil

^[5] Bergen Univ Coll, Inst Physiotherapy, N-5009 Bergen - Norway

^[6] Univ Bergen, Sect Physiotherapy Sci, Inst Publ Hlth & Primary Hlth Care, N-5018 Bergen - Norway

^[7] CHU Poitiers, Serv Oncol Radiotherap, F-86021 Poitiers - France

• Tipo de documento: Artigo Científico
• Fonte: BMC CANCER; v. 9, NOV 20 2009.
• Citações Web of Science: 29

Resumo

Background: It has been speculated that the biostimulatory effect of Low Level Laser Therapy could cause undesirable enhancement of tumor growth in neoplastic diseases. The aim of the present study is to analyze the behavior of melanoma cells (B16F10) in vitro and the in vivo development of melanoma in mice after laser irradiation. Methods: We performed a controlled in vitro study on B16F10 melanoma cells to investigate cell viability and cell cycle changes by the Tripan Blue, MTT and cell quest histogram tests at 24, 48 and 72 h post irradiation. The in vivo mouse model (male Balb C, n = 21) of melanoma was used to analyze tumor volume and histological characteristics. Laser irradiation was performed three times (once a day for three consecutive days) with a 660 nm 50 mW CW laser, beam spot size 2 mm(2), irradiance 2.5 W/cm(2) and irradiation times of 60s (dose 150 J/cm(2)) and 420s (dose 1050 J/cm(2)) respectively. Results: There were no statistically significant differences between the in vitro groups, except for an increase in the hypodiploid melanoma cells (8.48 +/- 1.40% and 4.26 +/- 0.60%) at 72 h postirradiation. This cancer-protective effect was not reproduced in the in vivo experiment where outcome measures for the 150 J/cm(2) dose group were not significantly different from controls. For the 1050 J/cm(2) dose group, there were significant increases in tumor volume, blood vessels and cell abnormalities compared to the other groups. Conclusion: LLLT Irradiation should be avoided over melanomas as the combination of high irradiance (2.5 W/cm(2)) and high dose (1050 J/cm(2)) significantly increases melanoma tumor growth in vivo. (AU)

• Processo FAPESP: 07/59124-0 - Avaliação dos efeitos da terapia fotodinâmica com azul de metileno no modelo experimental de ratos com tumor de Walker 256
• Beneficiário: Lúcio Frigo
• Modalidade de apoio: Auxílio à Pesquisa - Regular