A key role for Na+/K+-ATPase in the endothelium-dependent oscillatory activity of mouse small mesenteric arteries

Autor(es)

Giachini, F. R. C. ^{[1, 2]} ; Carneiro, F. S. ^{[1, 2]} ; Lima, V. V. ^{[1, 2]} ; Carneiro, Z. N. ^[1] ; Brands, M. W. ^[1] ; Webb, R. C. ^[1] ; Tostes, R. C. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Med Coll Georgia, Dept Physiol, Augusta, GA 30912 - USA

^[2] Univ Sao Paulo, Fac Med, Dept Farmacol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Medical and Biological Research; v. 42, n. 11, p. 1058-1067, NOV 2009.
• Citações Web of Science: 5

Resumo

Oscillatory contractile activity is an inherent property of blood vessels. Various cellular mechanisms have been proposed to contribute to oscillatory activity. Mouse small mesenteric arteries display a unique low frequency contractile oscillatory activity (1 cycle every 10-12 min) upon phenylephrine stimulation. Our objective was to identify mechanisms involved in this peculiar oscillatory activity. First-order mesenteric arteries were mounted in tissue baths for isometric force measurement. The oscillatory activity was observed only in vessels with endothelium, but it was not blocked by L-NAME (100 µM) or indomethacin (10 µM), ruling out the participation of nitric oxide and prostacyclin, respectively, in this phenomenon. Oscillatory activity was not observed in vessels contracted with K+ (90 mM) or after stimulation with phenylephrine plus 10 mM K+. Ouabain (1 to 10 µM, an Na+/K+-ATPase inhibitor), but not K+ channel antagonists [tetraethylammonium (100 µM, a nonselective K+ channel blocker), Tram-34 (10 µM, blocker of intermediate conductance K+ channels) or UCL-1684 (0.1 µM, a small conductance K+ channel blocker)], inhibited the oscillatory activity. The contractile activity was also abolished when experiments were performed at 20°C or in K+-free medium. Taken together, these results demonstrate that Na+/K+-ATPase is a potential source of these oscillations. The presence of α-1 and α-2 Na+/K+-ATPase isoforms was confirmed in murine mesenteric arteries by Western blot. Chronic infusion of mice with ouabain did not abolish oscillatory contraction, but up-regulated vascular Na+/K+-ATPase expression and increased blood pressure. Together, these observations suggest that the Na+/K+ pump plays a major role in the oscillatory activity of murine small mesenteric arteries. (AU)

• Processo FAPESP: 06/01773-0 - Sistema de sinalização RhoA/Rho-cinase no músculo liso vascular de camundongos com hipertensão induzida por angiotensina II: interação com espécies reativas de oxigênio, interleucina-6 (IL-6) e via de sinalização JAK/STAT
• Beneficiário: Rita de Cassia Aleixo Tostes Passaglia
• Modalidade de apoio: Bolsas no Exterior - Novas Fronteiras