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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Nitric oxide detection in cell culture exposed to LPS after Er:YAG laser irradiation

Texto completo
Autor(es):
Rocha, R. A. S. S. [1] ; Silva, R. A. B. [1] ; Assed, S. [1] ; Medeiros, A. I. [2] ; Faccioli, L. H. [2] ; Pecora, J. D. [3] ; Nelson-Filho, P. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dent Sch Ribeirao Preto, Dept Paediat Prevent & Community Dent, BR-14040904 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Dept Clin Toxicol & Bromatol Anal, Sch Pharmaceut Sci Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Dept Restorat Dent, Dent Sch Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: International Endodontic Journal; v. 42, n. 11, p. 992-996, NOV 2009.
Citações Web of Science: 6
Resumo

P>Aim To evaluate in vitro the effect of calcium hydroxide {[}Ca(OH)(2)] and Er:YAG laser on bacterial endotoxin {[}also known as lipopolysaccharide (LPS)] as determined by nitric oxide (NO) detection in J774 murine macrophage cell line culture. Methodology Samples of LPS solution (50 mu gmL-1), Ca(OH)(2) suspension (25 mg mL-1) and LPS suspension with Ca(OH)(2) were prepared. The studied groups were: I - LPS (control); II - LPS + Ca(OH)(2); III - LPS + Er:YAG laser (15 Hz 140 mJ); IV - LPS + Er:YAG laser (15 Hz 200 mJ); V - LPS + Er:YAG laser (15 Hz 250 mJ), VI - Pyrogen-free water; VII - Ca(OH)(2). Murine macrophage J774 cells were plated and 10 mu L of the samples were added to each well. The supernatants were collected for NO detection by the Griess reaction. Data were analysed statistically by one-way anova and Tukey's test at 5% significance level. Results The mean and SE (in mu mol L-1) values of NO release were: I - 10.48 +/- 0.58, II - 6.41 +/- 0.90, III - 10.2 +/- 0.60, IV - 8.35 +/- 0.40, V - 10.40 +/- 0.53, VI - 3.75 +/- 0.70, VII - 6.44 +/- 0.60; and the values for the same experiment repeated after 1 week were: I - 21.20 +/- 1.50, II - 9.10 +/- 0.60, III - 19.50 +/- 1.00, IV - 18.50 +/- 0.60, V - 21.30 +/- 0.90, VI - 2.00 +/- 0.20, VII - 6.80 +/- 1.70. There was no significant difference (P > 0.05) between the control and the laser-treated groups (III, IV and V), or comparing groups II, VI and VII to each other (P > 0.05). Group I had significantly higher NO release than group II (P < 0.05). Groups II and VI had similar NO release (P > 0.05). Conclusions Calcium hydroxide inactivated the bacterial endotoxin (LPS) whereas none of the Er:YAG laser parameter settings had the same effectiveness. (AU)

Processo FAPESP: 02/12856-2 - Modulação das respostas imunes inata e adquirida por leucotrienos e prostaglandinas
Beneficiário:Lúcia Helena Faccioli
Linha de fomento: Auxílio à Pesquisa - Temático