| Texto completo | |
| Autor(es): |
Feitosa, Weber Beringui
[1]
;
Milazzotto, Marcella Pecora
[1, 2]
;
Simoes, Renata
[1]
;
Rovegno, Mariana
[1]
;
Nicacio, Alessandra Coralo
[1, 3]
;
Nascimento, Anibal Ballarotti
[4, 1]
;
Arruda Goncalves, Jose Sergio
[1]
;
Visintin, Jose Antonio
[1]
;
Ortiz D'Avila Assumpcao, Mayra Elena
[1]
Número total de Autores: 9
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Dept Anim Reprod, Coll Vet Med & Anim Sci, BR-05508900 Sao Paulo - Brazil
[2] Fed Univ ABC, Ctr Nat Human Sci, Santo Andre - Brazil
[3] Univ Estadual Maranhao, Sao Luis - Brazil
[4] Univ Wisconsin, Dept Dairy Sci, Madison, WI 53706 - USA
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ZYGOTE; v. 17, n. 4, p. 315-320, NOV 2009. |
| Citações Web of Science: | 11 |
| Resumo | |
The aim of this study was to assess the effect of exogenous DNA and incubation time on the viability of bovine sperm. Sperm were incubated at a concentration of 5 x 10(6)/ml with or without plasmid pEYFP-NUC. Fluorescent probes, propidium iodide/Hoechst 33342, FITC-PSA and JC-1, were used to assess plasma membrane integrity (PMI), acrosome membrane integrity (AMI) and mitochondrial membrane potential (MMP) respectively at 0, 1, 2, 3 and 4 h of incubation. Exogenous DNA addition did not affect sperm viability; however, incubation time was related to sperm deterioration. Simultaneous assessment of PMI, AMI and MMP showed a reduction in the number of sperm with higher viability (integrity of plasma and acrosome membranes and high mitochondrial membrane potential) from 58.7% at 0 h to 7.5% after 4 h of incubation. Lower viability sperm (damaged plasma and acrosome membranes and low mitochondrial membrane potential) increased from 4.6% at 0 h to 25.99% after 4 h of incubation. When PMI, AMI and MMP were assessed separately we noticed a reduction in plasma and acrosome membrane integrity and mitochondrial membrane potential throughout the incubation period. Therefore, exogenous DNA addition does not affect sperm viability, but the viability is reduced by incubation time. (AU) | |
| Processo FAPESP: | 03/10234-7 - Espermatozoide bovino como vetor de transgene: efeito da estrutura e do tamanho do dna exogeno na internalizacao no nucleo do espermatozoide. |
| Beneficiário: | Weber Beringui Feitosa |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 03/07456-8 - Estudo da integracao do dna exogeno em espermatozoides de bovinos. |
| Beneficiário: | Mayra Elena Ortiz D' Avila Assumpcao |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |