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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bovine sperm cells viability during incubation with or without exogenous DNA

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Author(s):
Feitosa, Weber Beringui [1] ; Milazzotto, Marcella Pecora [1, 2] ; Simoes, Renata [1] ; Rovegno, Mariana [1] ; Nicacio, Alessandra Coralo [1, 3] ; Nascimento, Anibal Ballarotti [4, 1] ; Arruda Goncalves, Jose Sergio [1] ; Visintin, Jose Antonio [1] ; Ortiz D'Avila Assumpcao, Mayra Elena [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Dept Anim Reprod, Coll Vet Med & Anim Sci, BR-05508900 Sao Paulo - Brazil
[2] Fed Univ ABC, Ctr Nat Human Sci, Santo Andre - Brazil
[3] Univ Estadual Maranhao, Sao Luis - Brazil
[4] Univ Wisconsin, Dept Dairy Sci, Madison, WI 53706 - USA
Total Affiliations: 4
Document type: Journal article
Source: ZYGOTE; v. 17, n. 4, p. 315-320, NOV 2009.
Web of Science Citations: 11
Abstract

The aim of this study was to assess the effect of exogenous DNA and incubation time on the viability of bovine sperm. Sperm were incubated at a concentration of 5 x 10(6)/ml with or without plasmid pEYFP-NUC. Fluorescent probes, propidium iodide/Hoechst 33342, FITC-PSA and JC-1, were used to assess plasma membrane integrity (PMI), acrosome membrane integrity (AMI) and mitochondrial membrane potential (MMP) respectively at 0, 1, 2, 3 and 4 h of incubation. Exogenous DNA addition did not affect sperm viability; however, incubation time was related to sperm deterioration. Simultaneous assessment of PMI, AMI and MMP showed a reduction in the number of sperm with higher viability (integrity of plasma and acrosome membranes and high mitochondrial membrane potential) from 58.7% at 0 h to 7.5% after 4 h of incubation. Lower viability sperm (damaged plasma and acrosome membranes and low mitochondrial membrane potential) increased from 4.6% at 0 h to 25.99% after 4 h of incubation. When PMI, AMI and MMP were assessed separately we noticed a reduction in plasma and acrosome membrane integrity and mitochondrial membrane potential throughout the incubation period. Therefore, exogenous DNA addition does not affect sperm viability, but the viability is reduced by incubation time. (AU)