Role of spinal microglia in myositis-induced central sensitisation: An immunohistochemical and behavioural study in rats

Autor(es)

Chacur, Marucia ^{[1, 2]} ; Lambertz, Daniela ^[2] ; Hoheisel, Ulrich ^[3] ; Mense, Siegfried ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05508900 Sao Paulo - Brazil

^[2] Univ Heidelberg, Inst Anat & Cell Biol, D-69120 Heidelberg - Germany

^[3] Humboldt Univ, Charite, Inst Pharmacol & Toxicol, D-10117 Berlin - Germany

• Tipo de documento: Artigo Científico
• Fonte: EUROPEAN JOURNAL OF PAIN; v. 13, n. 9, p. 915-923, OCT 2009.
• Citações Web of Science: 27

Resumo

There is increasing evidence that spinal glial cells play an important role in chronic pain states. However, so far no data on the role of microglia in muscle pain are available. The aim of the present study was to investigate the involvement of spinal microglial cells in chronic muscle pain. In a rat model of chronic muscle inflammation (injection of complete Freunds adjuvant into the gastrocnemius-soleus muscle) alterations of microglia were visualized with quantitative OX-42 immunohistochemistry in the dorsal horn of the segments L4 and L5 12 days after induction of inflammation. In behavioural experiments the influence of chronic intrathecally applied minocycline - a specific microglia inhibitor - or an antibody against tumour necrosis factor-alpha (TNF-alpha: a cytokine released from microglia) on pain-related behaviour was investigated after 1, 3, 6, and 12 days. The immunhistochemical data show that in the deep laminae of the spinal dorsal horn microglial cells reacted with morphological changes to the muscle inflammation. Following inflammation, the mean boundary length surrounding the OX-42 immunostained area was significantly shorter. This indicates that microglial cells were activated by the myositis and withdrew their processes. Chronic intrathecal administration of minocycline or anti TNF-alpha with an osmotic mini-pump largely normalised the inflammation-induced changes in spontaneous exploratory behaviour and attenuated the hypersensitivity to mechanical stimulation. Both the immunohistochemical and behavioural data show that spinal microglial cells are involved in nociceptive processes in the cause of a chronic muscle inflammation. (C) 2008 European Federation of International Association for the Study of Pain Chapters. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 06/57423-7 - Eletrofisiologia de neurônios do corno dorsal da medula espinhal: participação de células da glia em modelo de inflamação periférica
• Beneficiário: Marucia Chacur
• Modalidade de apoio: Bolsas no Exterior - Pesquisa