Evaluation of Toxoplasma gondii placental transmission in BALB/c mice model

Autor(es)

Pezerico, Sandia B. ^[1] ; Langoni, Helio ^{[2, 1]} ; Da Silva, Aristeu V. ^[3] ; Da Silva, Rodrigo C.

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Fac Med Botucatu, BR-18618000 Botucatu, SP - Brazil

^[2] Univ Estadual Paulista, Fac Med Vet & Zootecnia, Dept Higiene Vet & Saude Publ, BR-18618000 Botucatu, SP - Brazil

^[3] Univ Paranaense, BR-87502210 Umuarama, PR - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Experimental Parasitology; v. 123, n. 2, p. 168-172, OCT 2009.
• Citações Web of Science: 10

Resumo

Toxoplasma gondii infection is common worldwide and highly important to pregnant women as it can be transmitted to the fetus via the placenta. This study aimed at evaluating the prevention of placental transmission in two different strains after chronic infection with each one of the strains. A BALB/c mice model was inoculated 30 days before breeding (immunization) and re-infected 12 and 15 days after pregnancy (challenge). Seven experimental groups were assayed: G1: ME49-immunization (type II), M7741-challenge (type III); G2: M7741-immunization, ME49-challenge; G3, ME49-immunization; G4: M7741-immunization; G5: ME49-challenge; G6: M7741-challenge; G7: saline solution inoculation. Serology, mouse bioassay, PCR and RLFP of the uterus, placenta and fetus were performed to determine the congenital transmission of the strains challenged after chronic infection. IgG T. gondii antibodies were detected in G1, G2, G3 and G4, but not in G5, G6 and G7. All animals of G5 and G6 were IgM-positive. Congenital infection was not detected by bioassay and PCR. Nonetheless, placentas from G3 and G4 resulted positive but no corresponding fetal infection was detected. G1 and G2 did not show the genotype of the strain challenged during pregnancy, only those of chronic infection. Thus, the chronically infected BALB/c mice showed no re-infection after inoculation with another strain during pregnancy. Further studies with different parasite loads and different mice lineages are needed. (C) 2009 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 01/12052-8 - Avaliação da transmissão transplacentária de cepas de Toxoplasma gondii genotipicamente distintas, em camundongas BALB/c
• Beneficiário: Sandia Bergamaschi Pezerico
• Modalidade de apoio: Bolsas no Brasil - Mestrado