Microsatellite diversity and effective population size in a germplasm bank of Hymenaea courbaril var. stilbocarpa (Leguminosae), an endangered tropical tree: recommendations for conservation

Autor(es)

Feres, Juliana Massimino ^{[1, 2]} ; Guidugli, Marcela Corbo ^{[1, 3]} ; Mestriner, Moacyr Antonio ^[1] ; Sebbenn, Alexandre Magno ^[4] ; Ciampi, Ana Yamaguishi ^[5] ; Alzate-Marin, Ana Lilia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Plant Genet Lab, Dept Genet, FMRP, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dept Genet, Grad Program Genet, FMRP, BR-14049900 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Grad Program Comparat Biol, Dept Biol, FFCLRP, BR-14040900 Ribeirao Preto, SP - Brazil

^[4] Forest Inst Sao Paulo, Expt Stn Tupi, BR-13400970 Piracicaba, SP - Brazil

^[5] PqEB, Embrapa Cenargen, BR-70770900 Brasilia, DF - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Genetic Resources and Crop Evolution; v. 56, n. 6, p. 797-807, SEP 2009.
• Citações Web of Science: 7

Resumo

Deforestation in southeast Brazil has led to the extinction of Hymenaea courbaril var. stilbocarpa and ex situ conservation has been established. In this study, the levels of genetic diversity and the effective population size of H. courbaril in a germplasm bank were investigated using six nuclear microsatellite loci. A total of 79 and 91 alleles were found in 65 seed-trees and their 176 offspring, respectively. Offspring have a higher average number of alleles per locus (A = 15.2) than seed-trees (A = 13.2), but lower observed heterozygosity (offspring: H (o) = 0.566; seed-trees: H (o) = 0.607). The estimate of outcrossing rate shows that the study population is perfectly outcrossed (t (m) = 0.978, P > 0.05). Significant deviations from random mating were detected through mating among relatives and correlated matings. The average variance in effective population size for each family was 2.63, with a total effective population size retained in the bank of 170.1. These results confirm that the preserved population of H. courbaril retains substantial genetic variability. (AU)

• Processo FAPESP: 03/04199-4 - Uso de marcadores SSR na análise genética de árvores matrizes vs. populações derivadas que formam parte do Banco de Germoplasma da Floresta da USP-RP
• Beneficiário: Ana Lilia Alzate Marin
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores