Effects of the domestic use of a disclosing solution on the denture biofilm: a preliminary study

Autor(es)

de Souza, R. F. ^[1] ; Nascimento, C. ^[1] ; Regis, R. R. ^[1] ; Silva-Lovato, C. H. ^[1] ; Paranhos, H. F. O. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Dent Mat & Prosthodont, Ribeirao Preto Sch Dent, BR-14049 Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Oral Rehabilitation; v. 36, n. 7, p. 491-497, JUL 2009.
• Citações Web of Science: 5

Resumo

To investigate the effect of the home use of a disclosing agent on the microbial composition of denture biofilm, by means of a cross-over randomized clinical trial. Two interventions were tested during 7 days each: (i) oral and denture hygiene instructions and (ii) instructions associated with the home use of a disclosing agent (1% neutral red). Eleven participants with visible biofilm deposits over their maxillary complete dentures were randomly assigned to one of the two sequences of interventions: (i) I followed by II, and (ii) II followed by I. A washout period of 7 days was established. After each intervention, samples of denture biofilm were evaluated by DNA checkerboard hybridization for the detection of Candida spp. and 17 bacterial species. Counts were low for all the tested species, and no significant difference was found between the tested interventions ( Wilcoxon test, P > 0.05). The home use of a disclosing agent does not remarkably change the composition of denture biofilm. (AU)

• Processo FAPESP: 06/56194-4 - Estudo randomizado e controlado do uso caseiro de um evidenciador de biofilme sobre a higiene de proteses totais.
• Beneficiário: Raphael Freitas de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Regular