Interactions of mast cell degranulating peptides with model membranes: A comparative biophysical study

Autor(es)

dos Santos Cabrera, Marcia Perez ^{[1, 2]} ; Arcisio-Miranda, Manoel ^[1] ; da Costa, Laiana Cristina ^[2] ; de Souza, Bibiana Monson ^[3] ; Broggio Costa, Sabrina Thais ^[2] ; Palma, Mario Sergio ^[3] ; Ruggiero Neto, Joao ^[2] ; Procopio, Joaquim ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Physiol & Biophys, Inst Biomed Sci, Sao Paulo - Brazil

^[2] Sao Paulo State Univ, UNESP, IBILCE, Dept Phys, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[3] Sao Paulo State Univ, UNESP, Ctr Studies Social Insects, Inst Biosci, Rio Claro, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Archives of Biochemistry and Biophysics; v. 486, n. 1, p. 1-11, JUN 1 2009.
• Citações Web of Science: 21

Resumo

In the last decade, there has been renewed interest in biologically active peptides in fields like allergy, autoimmume diseases and antibiotic therapy. Mast cell degranulating peptides mimic G-protein receptors, showing different activity levels even among homologous peptides. Another important feature is their ability to interact directly with membrane phospholipids, in a fast and concentration-dependent way. The mechanism of action of peptide HR1 on model membranes was investigated comparatively to other mast cell degranulating peptides (Mastoparan, Eumenitin and Anoplin) to evidence the features that modulate their selectivity. Using vesicle leakage, single-channel recordings and zeta-potential measurements, we demonstrated that HR1 preferentially binds to anionic bilayers, accumulates, folds, and at very low concentrations, is able to insert and create membrane spanning ion-selective pores. We discuss the ion selectivity character of the pores based on the neutralization or screening of the peptides charges by the bilayer head group charges or dipoles. (C) 2009 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 06/57122-7 - Procura de compostos líderes para o desenvolvimento racional de novos fármacos e pesticidas a partir bioprospecção da fauna de artrópodes brasileiros
• Beneficiário: Mario Sergio Palma
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático