Bacterial community in the rhizosphere and rhizoplane of wild type and transgenic eucalyptus

Autor(es)

Andreote, Fernando D. ^{[1, 2]} ; Rossetto, Priscilla B. ^[1] ; Mendes, Rodrigo ^[1] ; Avila, Luciana A. ^[2] ; Labate, Carlos A. ^[1] ; Pizzirani-Kleiner, Aline A. ^[1] ; Azevedo, Joao L. ^[1] ; Araujo, Welington L. ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, BR-13400970 Piracicaba - Brazil

^[2] Embrapa Meio Ambiente, Lab Microbiol Ambiental, Jaguariuna - Brazil

^[3] Univ Mogi das Cruzes, Nucleo Integrado Biotecnol, Mogi Das Cruzes - Brazil

• Tipo de documento: Artigo Científico
• Fonte: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY; v. 25, n. 6, p. 1065-1073, JUN 2009.
• Citações Web of Science: 15

Resumo

The rhizosphere is a niche exploited by a wide variety of bacteria. The expression of heterologous genes by plants might become a factor affecting the structure of bacterial communities in the rhizosphere. In a greenhouse experiment, the bacterial community associated to transgenic eucalyptus, carrying the Lhcb1-2 genes from pea (responsible for a higher photosynthetic capacity), was evaluated. The culturable bacterial community associated to transgenic and wild type plants were not different in density, and the Amplified Ribosomal DNA Restriction Analysis (ARDRA) typing of 124 strains revealed dominant ribotypes representing the bacterial orders Burkholderiales, Rhizobiales, and Actinomycetales, the families Xanthomonadaceae, and Bacillaceae, and the genus Mycobacterium. Principal Component Analysis based on the fingerprints obtained by culture-independent Denaturing Gradient Gel Electrophoresis analysis revealed that Alphaproteobacteria, Betaproteobacteria and Actinobacteria communities responded differently to plant genotypes. Similar effects for the cultivation of transgenic eucalyptus to those observed when two genotype-distinct wild type plants are compared. (AU)

• Processo FAPESP: 02/14143-3 - Interação entre populações microbianas e plantas geneticamente modificadas
• Beneficiário: Welington Luiz de Araújo
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 03/01438-8 - Interação entre bactérias endofíticas e cana-de-açúcar geneticamente modificada (Saccharum spp.)
• Beneficiário: Priscilla de Barros Rossetto
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 00/14987-1 - Caracterização da comunidade bacteriana endofítica e análise da interação com Xylella fastidiosa
• Beneficiário: Paulo Teixeira Lacava
• Modalidade de apoio: Bolsas no Brasil - Doutorado