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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bacterial community in the rhizosphere and rhizoplane of wild type and transgenic eucalyptus

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Author(s):
Andreote, Fernando D. [1, 2] ; Rossetto, Priscilla B. [1] ; Mendes, Rodrigo [1] ; Avila, Luciana A. [2] ; Labate, Carlos A. [1] ; Pizzirani-Kleiner, Aline A. [1] ; Azevedo, Joao L. [1] ; Araujo, Welington L. [1, 3]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, BR-13400970 Piracicaba - Brazil
[2] Embrapa Meio Ambiente, Lab Microbiol Ambiental, Jaguariuna - Brazil
[3] Univ Mogi das Cruzes, Nucleo Integrado Biotecnol, Mogi Das Cruzes - Brazil
Total Affiliations: 3
Document type: Journal article
Source: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY; v. 25, n. 6, p. 1065-1073, JUN 2009.
Web of Science Citations: 15
Abstract

The rhizosphere is a niche exploited by a wide variety of bacteria. The expression of heterologous genes by plants might become a factor affecting the structure of bacterial communities in the rhizosphere. In a greenhouse experiment, the bacterial community associated to transgenic eucalyptus, carrying the Lhcb1-2 genes from pea (responsible for a higher photosynthetic capacity), was evaluated. The culturable bacterial community associated to transgenic and wild type plants were not different in density, and the Amplified Ribosomal DNA Restriction Analysis (ARDRA) typing of 124 strains revealed dominant ribotypes representing the bacterial orders Burkholderiales, Rhizobiales, and Actinomycetales, the families Xanthomonadaceae, and Bacillaceae, and the genus Mycobacterium. Principal Component Analysis based on the fingerprints obtained by culture-independent Denaturing Gradient Gel Electrophoresis analysis revealed that Alphaproteobacteria, Betaproteobacteria and Actinobacteria communities responded differently to plant genotypes. Similar effects for the cultivation of transgenic eucalyptus to those observed when two genotype-distinct wild type plants are compared. (AU)