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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

No-match ORESTES explored as tumor markers

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Autor(es):
Mello, Barbara P. [1] ; Abrantes, Eduardo F. [1] ; Torres, Cesar H. [1] ; Machado-Lima, Ariane [2] ; Fonseca, Rogrio da Silva [1] ; Carraro, Dirce M. [1] ; Brentani, Ricardo R. [1] ; Reis, Luiz F. L. [1] ; Brentani, Helena [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Hosp AC Camargo Fund Antonio Prudente, BR-01509900 Sao Paulo - Brazil
[2] Univ Sao Paulo, IPq, IME, BR-05508090 Sao Paulo - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Nucleic Acids Research; v. 37, n. 8, p. 2607-2617, MAY 2009.
Citações Web of Science: 9
Resumo

Sequencing technologies and new bioinformatics tools have led to the complete sequencing of various genomes. However, information regarding the human transcriptome and its annotation is yet to be completed. The Human Cancer Genome Project, using ORESTES (open reading frame EST sequences) methodology, contributed to this objective by generating data from about 1.2 million expressed sequence tags. Approximately 30 of these sequences did not align to ESTs in the public databases and were considered no-match ORESTES. On the basis that a set of these ESTs could represent new transcripts, we constructed a cDNA microarray. This platform was used to hybridize against 12 different normal or tumor tissues. We identified 3421 transcribed regions not associated with annotated transcripts, representing 83.3 of the platform. The total number of differentially expressed sequences was 1007. Also, 28 of analyzed sequences could represent noncoding RNAs. Our data reinforces the knowledge of the human genome being pervasively transcribed, and point out molecular marker candidates for different cancers. To reinforce our data, we confirmed, by real-time PCR, the differential expression of three out of eight potentially tumor markers in prostate tissues. Lists of 1007 differentially expressed sequences, and the 291 potentially noncoding tumor markers were provided. (AU)

Processo FAPESP: 07/01549-5 - Identificacao de rnas nao codificantes em transtornos neuropsiquiatricos.
Beneficiário:Ariane Machado Lima
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 04/11774-8 - Identificacao de novos genes humanos atraves da exploracao racional do banco de dados do projeto genoma do cancer humano (hcgp).
Beneficiário:Barbara Pereira de Mello
Modalidade de apoio: Bolsas no Brasil - Mestrado
Processo FAPESP: 07/55791-1 - Busca de marcadores moleculares tecido-associados em regioes transcritas nao caracterizadas do genoma humano.
Beneficiário:Barbara Pereira de Mello
Modalidade de apoio: Bolsas no Brasil - Doutorado