| Texto completo | |
| Autor(es): |
Luz, Juliana S.
[1]
;
Georg, Raphaela C.
[1]
;
Gomes, Carlos H.
[1]
;
Machado-Santelli, Glauci M.
[2]
;
Oliveira, Carla C.
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Cellular & Dev Biol, BR-05508000 Sao Paulo - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | YEAST; v. 26, n. 5, p. 287-298, MAY 2009. |
| Citações Web of Science: | 15 |
| Resumo | |
The Shwachman-Bodian-Diamond syndrome protein (SBDS) is a member of a highly conserved protein family of not well understood function, with putative orthologues found in different organisms ranging from Archaea, yeast and plants to vertebrate animals. The yeast orthologue of SBDS, Sdo1p, has been previously identified in association with the 60S ribosomal subunit and is proposed to participate in ribosomal recycling. Here we show that Sdo1p interacts with nucleolar rRNA processing factors and ribosomal proteins, indicating that it might bind the pre-60S complex and remain associated with it during processing and transport to the cytoplasm. Corroborating the protein interaction data, Sdo1p localizes to the nucleus and cytoplasm and co-immunoprecipitates precursors of 60S and 40S subunits, as well as the mature rRNAs. Sdo1p binds RNA directly, suggesting that it may associate with the ribosomal subunits also through RNA interaction. Copyright (C) 2009 John Wiley \& Sons, Ltd. (AU) | |
| Processo FAPESP: | 05/56493-9 - Caracterização funcional de uma nova proteína de Saccharomyces cerevisiae potencialmente envolvida em processamento de RNA |
| Beneficiário: | Carla Columbano de Oliveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |