Mass spectrometry fingerprinting of media used for in vitro production of bovine embryos

Autor(es)

Ferreira, Christina Ramires ^{[1, 2, 3]} ; Martins Ferreira Souza, Gustavo Henrique ^{[1, 4]} ; Riccio, Maria Francesca ^{[1, 5]} ; Catharino, Rodrigo Ramos ^{[1, 5]} ; Fortes Pontes, Jose Henrique ^[2] ; Basso, Andrea Cristina ^[2] ; Ereno Junior, Jose Carlos ^[2] ; Perecin, Felipe ^[3] ; Eberlin, Marcos Nogueira ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Quim, Lab ThoMSon Espectrometria Massas, BR-13083970 Campinas, SP - Brazil

^[2] In Vitro Brasil Ltda, BR-13800970 Mogi Mirim, SP - Brazil

^[3] USP, Dept Basic Sci, FZEA, Fac Anim Sci & Food Engn, BR-13635000 Pirassununga, SP - Brazil

^[4] Univ Estadual Campinas, Fac Med Sci, Dept Pharmacol, BR-13083970 Campinas, SP - Brazil

^[5] Univ Estadual Campinas, Sch Med Sci, Dept Clin Pathol, BR-13083887 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: RAPID COMMUNICATIONS IN MASS SPECTROMETRY; v. 23, n. 9, p. 1313-1320, MAY 2009.
• Citações Web of Science: 13

Resumo

Using the bovine species as a biological model, direct infusion chip-based nano-electrospray ionization mass spectrometry (nano-ESI-MS) fingerprinting in the positive ion mode is used to obtain fast chemical profiles of media used for in vitro production of bovine embryos. Nano-ESI-MS fingerprinting is useful for characterization and routine quality control requiring no sample preseparation, being able to differentiate four different media (IVM, IVF, SOF and HSOF) via principal component analysis (PCA). For media stored at +4 degrees C for up to 45 days, no significant (p>0.05) variation was observed in cleavage and blastocyst rate development, as well as in the nano-ESI-MS chemical profiles. For media exposed to a heat shock (60 degrees C for 3 h), no significant decrease (p > 0.05) in embryo development rates was observed, but nano-ESI-MS profiles were quite distant from fresh control media in the PCA. For frozen media (-70 degrees C for 2 months), again no significant variation (p>0.05) in embryo development was noticed, but nano-ESI-MS profiles from all media were significantly affected. These results indicate that nano-ESI(+)-MS fingerprinting was able to characterize different media based on their specific chemical profile. The technique seems therefore applicable as a routine quality control assay, detecting, for example, compositional changes after temperature variations that may affect post-transfer embryo viability. Copyright (C) 2009 John Wiley \& Sons, Ltd. (AU)

• Processo FAPESP: 07/01400-1 - Utilização da espectrometria de massas para controle de qualidade de meios de cultivo e do soro fetal bovino utilizado para a produção in vitro de embriões bovinos
• Beneficiário: Christina Ramires Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisa Inovativa em Pequenas Empresas - PIPE