Low doses of monocrotaline in rats cause diminished bone marrow cellularity and compromised nitric oxide production by peritoneal macrophages

Autor(es)

Hueza, Isis M. ^[1] ; Benassi, Julia C. ^[1] ; Raspantini, Paulo C. F. ^[1] ; Raspantini, Leonila E. R. ^[1] ; Sa, Lilian R. M. ^[1] ; Gorniak, Silvana L. ^[1] ; Haraguchi, Mitsue ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Pathol, Sch Vet Med & Anim Sci, Res Ctr Vet Toxicol CEPTOX, BR-05508900 Sao Paulo - Brazil

^[2] Biol Inst Sao Paulo, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF IMMUNOTOXICOLOGY; v. 6, n. 1, p. 11-18, 2009.
• Citações Web of Science: 2

Resumo

Monocrotaline (MCT) is a pyrrolizidine alkaloid found in a variety of plants. The main symptoms of MCT toxicosis in livestock are related to hepato- and nephrotoxicity; in rodents and humans, the induction of a pulmonary hypertensive state that progresses to cor pulmonale has received much attention. Although studies have shown that MCT can cause effects on cellular functions that would be critical to those of lymphocytes/macrophages during a normal immune response, no immunotoxicological study on MCT have yet to ever be performed. Thus, the aim of the present study was to evaluate the effect of MCT on different branches of the immune system using the rat - which is known to be sensitive to the effects of MCT - as the model. Rats were treated once a day by gavage with 0.0, 0.3, 1.0, 3.0, or 5.0 mg MCT/kg for 14 days, and then any effects of the alkaloid on lymphoid organs, acquired immune responses, and macrophage activity were evaluated. No alterations in the relative weight of lymphoid organs were observed; however, diminished bone marrow cellularity in rats treated with the alkaloid was observed. MCT did not affect humoral or cellular immune responses. When macrophages were evaluated, treatments with MCT caused no significant alterations in phagocytic function or in hydrogen peroxide (H(2)O(2)) production; however, the MCT did cause compromised nitric oxide (NO) release by these cells. (AU)

• Processo FAPESP: 07/51648-0 - Avaliacao dos possiveis efeitos toxicos da monocrotalina sobre a resposta imune inata de ratos adultos e da prole de ratas tratadas durante a gestacao.
• Beneficiário: Julia Cristina Benassi
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 06/60397-8 - Toxinas de importância em medicina veterinária: estudos de imunotoxicidade e teratogenicidade: avaliação da imunotoxicidade, neurotoxicicidade e teratogenicidade em roedores e caprinos
• Beneficiário: Silvana Lima Gorniak
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 06/57174-7 - Desenvolvimento e validação de metodologia para avaliação de imunoteratologia de agentes tóxicos de interesse humano e veterinário
• Beneficiário: Isis Machado Hueza
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado