Photodynamic antimicrobial chemotherapy (PACT) with methylene blue increases membrane permeability in Candida albicans

Autor(es)

Giroldo, Ligia Maria ^[1] ; Felipe, Monalisa Poliana ^[1] ; de Oliveira, Marco Antonio ^[1] ; Munin, Egberto ^[1] ; Alves, Leandro Procopio ^[1] ; Costa, Maricilia Silva ^[1]

Afiliação do(s) autor(es)

^[1] Univ Vale Paraiba UNIVAP, IP&D, BR-12244000 Sao Jose Dos Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 24, n. 1, p. 109-112, JAN 2009.
• Citações Web of Science: 88

Resumo

Photodynamic antimicrobial chemotherapy (PACT) is a potential antimicrobial therapy that combines light and a photosensitizing drug, promoting a phototoxic effect on the treated cells, in general via oxidative damage. In this work we studied the effect of PACT, using methylene blue (MB), on the permeability of Candida albicans membrane. Our results demonstrated that the combination of MB and laser (684 nm) promoted a decrease in Candida growth. The inhibition was more pronounced in the presence of 0.05 mg/ml MB and with an energy density of 28 J/cm(2). The decrease in Candida growth was associated with an increase in membrane permeabilization. Thus, we suggest that a PACT mechanism using MB can be related to damage in the plasma membranes of the cells. (AU)

• Processo FAPESP: 04/10539-5 - Estudo da bioestimulação e da terapia fotodinâmica em Candida albicans
• Beneficiário: Maricilia Silva Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 01/12754-2 - Desenvolvimento de tecnicas opticas para caracterizacao de alteracoes induzidas por irradiacao laser em organismos de elevada capacidade regenerativa.
• Beneficiário: Egberto Munin
• Modalidade de apoio: Auxílio à Pesquisa - Regular