Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Expression of Sara2 human gene in erythroid progenitors

Texto completo
Autor(es):
Jardim, Denis Leonardo Fontes ; Cunha, Anderson Ferreira da ; Duarte, Adriana da Silva Santos ; Santos, Camila Oresco dos ; Saad, Sara Terezinha Olalla ; Costa, Fernando Ferreira [6]
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: Journal of Biochemistry and Molecular Biology; v. 38, n. 3, p. 328-333, May 2005.
Área do conhecimento: Ciências Biológicas - Genética
Assunto(s):Genética médica   Expressão gênica   Proteínas   Hemoglobinopatias   Terapia genética
Resumo

A human homologue of Sar1, named Sara2, was shown to be preferentially expressed during erythropoiesis in a culture stimulated by EPO. Previous studies, in yeast, have shown that secretion-associated and Ras-related protein (Sar1p) plays an essential role in protein transport from the endoplasmic reticulum to the Golgi apparatus. Here, we report the molecular analysis of Sara2 in erythroid cell culture. A 1250 bp long cDNA, encoding a 198 amino-acid protein very similar to Sar1 proteins from other organisms, was obtained. Furthermore, we also report a functional study of Sara2 with Real-time quantitative PCR analysis, demonstrating that expression of Sara2 mRNA increases during the initial stages of erythroid differentiation with EPO and that a two-fold increase in expression occurs following the addition of hydroxyurea (HU). In K562 cells, Sara2 mRNA was observed to have a constant expression and the addition of HU also up-regulated the expression in these cells. Our results suggest that Sara2 is an important gene in processes involving proliferation and differentiation and could be valuable for understanding the vesicular transport system during erythropoiesis. (AU)

Processo FAPESP: 02/13801-7 - Alterações hereditárias das hemoglobinas: genética molecular, aspectos da evolução clínica e produção de animais transgênicos
Beneficiário:Fernando Ferreira Costa
Linha de fomento: Auxílio à Pesquisa - Temático