| Texto completo | |
| Autor(es): |
Rezende, A. C. S.
[1]
;
Vieira, A. S.
[1]
;
Rogerio, F.
[1]
;
Rezende, L. F.
[1]
;
Boschero, A. C.
[1]
;
Negro, A.
[2]
;
Langone, F.
[1]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Campinas, Inst Biol, Dept Fisiol & Biofis, BR-13083970 Campinas, SP - Brazil
[2] Univ Padua, Dept Biochem, Ctr Ric Interdipartimentale Biotecnol Innovat, Padua, PD - Italy
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Brazilian Journal of Medical and Biological Research; v. 41, n. 11, p. 1024-1028, NOV 2008. |
| Citações Web of Science: | 13 |
| Resumo | |
Ciliary neurotrophic factor (CNTF) is a cytokine that plays a neuroprotective role in relation to axotomized motoneurons. We determined the effect of daily subcutaneous doses of CNTF (1.2 µg/g for 5 days; N = 13) or PBS (N = 13) on the levels of mRNA for Bcl-2 and Bax, as well as the expression and inter-association of Bcl-2 and Bax proteins, and the survival of motoneurons in the spinal cord lumbar enlargement of 2-day-old Wistar rats after sciatic nerve transection. Five days after transection, the effects were evaluated on histological and molecular levels using Nissl staining, immunoprecipitation, Western blot analysis, and reverse transcriptase-polymerase chain reaction. The motoneuron survival ratio, defined as the ratio between the number of motoneurons counted on the lesioned side vs those on the unlesioned side, was calculated. This ratio was 0.77 ± 0.02 for CNTF-treated rats vs 0.53 ± 0.02 for the PBS-treated controls (P < 0.001). Treatment with CNTF modified the level of mRNA, with the expression of Bax RNA decreasing 18% (with a consequent decrease in the level of Bax protein), while the expression of Bcl-2 RNA was increased 87%, although the level of Bcl-2 protein was unchanged. The amount of Bcl-2/Bax heterodimer increased 91% over that found in the PBS-treated controls. These data show, for the first time, that the neuroprotective effect of CNTF on neonatal rat axotomized motoneurons is associated with a reduction in free Bax, due to the inhibition of Bax expression, as well as increased Bcl-2/Bax heterodimerization. Thus, the neuroprotective action of the CNTF on axotomized motoneurons can be related to the inhibition of this apoptotic pathway. (AU) | |
| Processo FAPESP: | 05/54613-7 - Investigação da ação neuroprotetora do fator neurotrófico ciliar (CNTF) conjugado com o peptídio contendo domínio de translocação de proteína (PDT) |
| Beneficiário: | Francesco Langone |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 04/14210-8 - Investigacao da acao neuroprotetora do fator neurotrofico ciliar (cntf) conjugado com peptideo contendo dominio de translocacao de proteina (pdt). |
| Beneficiário: | Alexandre Cesar Santos de Rezende |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 05/51335-6 - Investigação dos efeitos da aplicação intracerebroventricular do fator neurotrófico ciliar (CNTF) conjugado com o peptídeo contendo domínio de translocação de proteína (PTD) sobre a via de sinalização JAK2/STAT3 no hipotálamo e suas implicações no tecido adiposo |
| Beneficiário: | Andre Schwambach Vieira |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 04/09786-8 - Fator neurotrófico ciliar (CNTF) e maturação da resposta secretória de Ilhotas de Langerhans de ratos recém-nascidos |
| Beneficiário: | Luiz Fernando de Rezende |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |