Immunohistochemical analysis of neuron types in the mouse small intestine

Autor(es)

Qu, Zheng-Dong ^{[1, 2]} ; Thacker, Michelle ^{[1, 2]} ; Castelucci, Patricia ^{[3, 1, 2]} ; Bagyanszki, Maria ^{[1, 2, 4]} ; Epstein, Miles L. ^[5] ; Furness, John B. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Melbourne, Dept Anat & Cell Biol, Parkville, Vic 3010 - Australia

^[2] Univ Melbourne, Ctr Neurosci, Parkville, Vic 3010 - Australia

^[3] Univ Sao Paulo, Dept Anat, Sao Paulo - Brazil

^[4] Univ Szeged, Dept Physiol Anat & Neurosci, H-6726 Szeged - Hungary

^[5] Univ Wisconsin, Sch Med, Dept Anat, Madison, WI 53706 - USA

• Tipo de documento: Artigo Científico
• Fonte: Cell and Tissue Research; v. 334, n. 2, p. 147-161, NOV 2008.
• Citações Web of Science: 156

Resumo

The definition of the nerve cell types of the myenteric plexus of the mouse small intestine has become important, as more researchers turn to the use of mice with genetic mutations to analyze roles of specific genes and their products in enteric nervous system function and to investigate animal models of disease. We have used a suite of antibodies to define neurons by their shapes, sizes, and neurochemistry in the myenteric plexus. Anti-Hu antibodies were used to reveal all nerve cells, and the major subpopulations were defined in relation to the Hu-positive neurons. Morphological Type II neurons, revealed by anti-neurofilament and anti-calcitonin gene-related peptide antibodies, represented 26% of neurons. The axons of the Type II neurons projected through the circular muscle and submucosa to the mucosa. The cell bodies were immunoreactive for choline acetyltransferase (ChAT), and their terminals were immunoreactive for vesicular acetylcholine transporter (VAChT). Nitric oxide synthase (NOS) occurred in 29% of nerve cells. Most were also immunoreactive for vasoactive intestinal peptide, but they were not tachykinin (TK)-immunoreactive, and only 10% were ChAT-immunoreactive. Numerous NOS terminals occurred in the circular muscle. We deduced that 90% of NOS neurons were inhibitory motor neurons to the muscle (26% of all neurons) and 10% (3% of all neurons) were interneurons. Calretinin immunoreactivity was found in a high proportion of neurons (52%). Many of these had TK immunoreactivity. Small calretinin neurons were identified as excitatory neurons to the longitudinal muscle (about 20% of neurons, with ChAT/calretinin/+/- TK chemical coding). Excitatory neurons to the circular muscle (about 10% of neurons) had the same coding. Calretinin immunoreactivity also occurred in a proportion of Type II neurons. Thus, over 90% of neurons in the myenteric plexus of the mouse small intestine can be currently identified by their neurochemistry and shape. (AU)

• Processo FAPESP: 06/57532-0 - Ageing, obesity and the survival of neurons in the intestine
• Beneficiário: Patricia Castelucci
• Modalidade de apoio: Bolsas no Exterior - Pesquisa