Classical and non-classical HLA molecules and p16(INK4a) expression in precursors lesions and invasive cervical cancer

Autor(es)

G. Goncalves, Maria Alice ^[1] ; Le Discorde, Magali ^[2] ; Simoes, Renata T. ; Rabreau, Michele ^[3] ; Soares, Edson G. ^[4] ; Donadi, Eduardo A. ; Carosella, Edgardo D. ^{[4, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Div Imunol Clin, Dept Clin Med, Hosp Clin, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Hop St Louis, CEA, Inst Univ Hematol, Serv Rech Hematoimmunol, DSV, DRM, F-75010 Paris - France

^[3] Inst Histocytopathol, F-33175 Le Bouscat - France

^[4] Univ Sao Paulo, Dept Pathol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY; v. 141, n. 1, p. 70-74, NOV 2008.
• Citações Web of Science: 27

Resumo

Objectives: Viruses and turnout cells may regulate the expression of HLA molecules on the cell surface to escape immune system surveillance. Absence of classical HLA class I molecules may impair the action of specific cytotoxic cells, whereas non-classical HLA class I molecules may regulate innate and adaptive immune cells. We assess here the possible associations between classical/non-classical class I HLA and p16(INK4a) molecule expression in cervical biopsies of women infected with HPV, stratified according to grade of the lesion and HPV type. Study design: Cervical biopsies (N = 74) presenting cervical intraepithelial neoplasia grade 1 (CIN1) (n = 31), CIN2-3 (n = 19), and invasive cancer (n = 14) were evaluated alongside 10 normal cervical specimens. Results: HLA-A/B/C/G staining was observed in the early stages of HPV infection. A significant association was detected between HLA-A/B/C staining and HPV16/18 infection (OR = 0.12, 95%CI: 0.0163-0.7899; p = 0.04). HLA-E expression increased with the progression of the lesion (chi(2)-test for trend = 4.01; p = 0.05), and a significant association was found between HLA-E staining and HPV16/18 infection (OR = 11.25, 95%CI: 2.324-54.465; p = 0.003). Irrespective of the grade of the lesion, HLA-A/B/C staining and p16(INK4a) presented a good concordance (Kappa: 0.67). Conclusions: HLA-E overexpression seemed to be associated with invasive cancer and HPV16/18 infection. (C) 2008 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 03/01019-5 - Expressão gênica do HLA-G em tecido cérvico-vaginal de mulheres infectadas pelo papilomavírus humano, segundo o polimorfismo do gene HLA-G
• Beneficiário: Maria Alice Guimarães Gonçalves
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Processo FAPESP: 01/02908-2 - Influência da imunodeficiência como fator de risco na patogênese das Neoplasias Intraepiteliais Cervicais (NIC)
• Beneficiário: Maria Alice Guimarães Gonçalves
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado