Platelet aggregation and TGF-beta(1) plasma levels in pregnant women with preeclampsia

Autor(es)

Serrao Peracoli, Maria Terezinha ^[1] ; Ferreira Menegon, Fernanda Tereza ^[2] ; Medeiros Borges, Vera Therezinha ^[2] ; de Araujo Costa, Roberto Antonio ^[2] ; Thomazini-Santos, Izolete Aparecida ^[3] ; Peracoli, Jose Carlos ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Botucatu Med Sch, Inst Biosci, Dept Microbiol & Immunol, BR-18618970 Botucatu, SP - Brazil

^[2] Sao Paulo State Univ, Botucatu Med Sch, Dept Obstet & Gynaecol, BR-18618970 Botucatu, SP - Brazil

^[3] Sao Paulo State Univ, Botucatu Med Sch, Haemostasy Lab Clin Hosp, BR-18618970 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF REPRODUCTIVE IMMUNOLOGY; v. 79, n. 1, p. 79-84, OCT 2008.
• Citações Web of Science: 28

Resumo

Objectives: Platelets and transforming growth factor-beta(1) (TGF-beta(1)) are thought to be involved in the pathogenesis of preeclampsia. Our objectives were to determine plasma concentration of TGF-beta(1) in normotensive and preeclamptic women in the third trimester of pregnancy and to evaluate the correlation of TGF-beta(1) plasma levels with platelet count and agonist-induced aggregation capacity. Methods: Thirty-three women with preeclampsia were compared with 36 healthy women with uncomplicated pregnancies. Peripheral venous blood samples were obtained, and TGF-beta(1) plasma levels measured by an enzyme-linked immunoassay. Platelet aggregation was induced by the agonist agents adenosine diphosphate (ADP), collagen and epinephrine, and was determined in platelet-rich plasma by aggregometry. Results: Plasma concentrations of active TGF-beta(1) were significantly higher in preeclamptic women (10.41 +/- 2.07 ng/mL) compared with normotensive pregnant women (7.01 +/- 3.29 ng/mL). Platelet number and platelet agonist-induced aggregation percent were significantly lower in patients with precclampsia than in healthy pregnant women. A significant correlation was observed between TGF-beta(1) plasma levels and platelet agonist-induced aggregation percent as between plasma levels of TGF-beta(1) and platelet number in preeclamptic patients. Conclusion: The association between impairment in platelet responsiveness and higher levels of TGF-beta(1) in the plasma of patients with preeclampsia suggests that this cytokine may play a role in the pathophysiological events of preeclampsia that are dependent on platelet activation. (C) 2008 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 02/09590-0 - Pre-eclampsia: compreensao da fisiopatologia e mecanismos de prevencao.
• Beneficiário: Jose Carlos Peraçoli
• Modalidade de apoio: Auxílio à Pesquisa - Regular