Genomovar status, virulence markers and genotyping of Burkholderia cepacia complex strains isolated from Brazilian cystic fibrosis patients

Autor(es)

Martins, Katia Maia ^[1] ; Fongaro, Giuliana F. ^[1] ; Dutra Rodrigues, Andre B. ^[2] ; Tateno, Adriana F. ^[2] ; Azzuz-Chernishev, Ana Carolina ^[3] ; de Oliveira-Garcia, Doroti ^[3] ; Rodrigues, Joaquim C. ^[1] ; da Silva Filho, Luiz Vicente F. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] HC FMUSP, Pediat Pulmonol Unit, Inst Crianca, BR-05450001 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Virol Lab, Inst Med Trop Sao Paulo, Sao Paulo - Brazil

^[3] Adolfo Lutz Inst, Bacteriol Sect, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Cystic Fibrosis; v. 7, n. 4, p. 336-339, JUL 2008.
• Citações Web of Science: 6

Resumo

Burkholderia cepacia complex isolates obtained by microbiological culture of respiratory samples from Brazilian CF patients were studied by recA based PCR, screened by specific PCR for virulence markers and genotyped by RAPD. Forty-one isolates of B. cepacia complex were identified by culture and confirmation of identity and genomovar determination obtained in 32 isolates, with predominance of B. cenocepacia (53.1%). Virulence markers were not consistently found among isolates. Genotyping did not identify identical patterns among different patients. B. cenocepacia was the most prevalent B. cepacia complex member among our patients, and cross-infection does not seem to occur among them. V 2008 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 02/13111-0 - Epidemiologia de bacilos gram negativos nao fermentadores de glicose isolados do trato respiratorio de pacientes portadores de fibrose cistica.
• Beneficiário: Doroti de Oliveira Garcia
• Modalidade de apoio: Auxílio à Pesquisa - Regular