Antileishmanial, antimalarial and antimicrobial activities of the extract and isolated compounds from Austroplenckia populnea (Celastraceae)

Autor(es)

Andrade, Sergio F. ^[1] ; da Silva Filho, Ademar A. ^[2] ; Resende, Dimas de O. ^[2] ; Silva, Marcio L. A. e ^[2] ; Cunha, Wilson R. ^[2] ; Nanayakkara, N. P. Dhammika ^[3] ; Bastos, Jairo Kenupp ^[4]

Afiliação do(s) autor(es)

^[1] Univ Vale Itajai UNIVALI, Nucl Invest Quim Farmaceut NIQFAR, Itajai, SC - Brazil

^[2] Univ Franca, Lab Quim Prod Nat, BR-14404600 Franca, SP - Brazil

^[3] Univ Mississippi, Natl Ctr Nat Prod Res, Oxford, MS - USA

^[4] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14049 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES; v. 63, n. 7-8, p. 497-502, JUL-AUG 2008.
• Citações Web of Science: 31

Resumo

Austroplenckia populnea (Celastraceae), known as ``marmelinho do campo{''}, is used in Brazilian folk medicine as antimicrobial, anti-inflammatory, and antitumoural agent. The aim of the present work was to evaluate the antimicrobial. antileishmanial and antimalarial activities of the crude hydroalcoholic extract of A. populnea (CHE) and some of its isolated compounds. The phytochemical study of the CHE was carried Out affording the isolation of methyl populnoate (1), populnoic acid (2), and stigmast-5-en-3-O-beta-(D-glucopyranoside) (3). This is the first time that the presence of compound 3 in A. populnea is reported. The results showed that the CHE presents antifungal and antibacterial activities, especially against Candida glabrata and Candida albicans, for which the CHE showed IC(50) values of 0.7 mu g mL(-1) and 5.5 mu g mL(-1), respectively, while amphotericin B showed an IC(50) value of 0.1 mu g mL(-1) against both microorganisms. Compounds 1-3 were inactive against all tested microorganisms. In the antileishmanial activity test against Leishmania donovani, the CHE showed an IC(50) value of 52 mu g mL(-1), while compounds 2 and 3 displayed an IC(50) value of 18 mu g mL(-1). In the antimalarial assay against Plasmodium falciparum (D6 and W2 clones), it was observed that all evaluated samples were inactive. In order to compare the effect on the parasites with the toxicity to mammalian cells, the cytotoxicity activity of the isolated compounds was evaluated against Vero cells, showing that all evaluated samples exhibited no cytotoxicity at the maximum dose tested. (AU)

• Processo FAPESP: 01/14219-7 - Estudo fitoquímico, realização de ensaios biológicos, cultivo e influência da sazonalidade no metabolismo secundário de Braccharis dracunculifolia e na produção de própolis verde por Apis mellifera
• Beneficiário: Jairo Kenupp Bastos
• Modalidade de apoio: Auxílio à Pesquisa - Regular