Genetic analysis of forest species Eugenia uniflora L. through of newly developed SSR markers

Autor(es)

Ferreira-Ramos, Ronai ^{[1, 2]} ; Laborda, Prianda R. ^[3] ; Santos, Melissa de Oliveira ^[3] ; Mayor, Matheus S. ^[1] ; Mestriner, Moacyr A. ^[1] ; de Souza, Anete P. ^[3] ; Alzate-Marin, Ana Lilia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Genet, Lab Genet Vegetal, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Programa Posgrad Biol Comparada, BR-14049 Ribeirao Preto, SP - Brazil

^[3] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CONSERVATION GENETICS; v. 9, n. 5, p. 1281-1285, 2008.
• Citações Web of Science: 12

Resumo

Nine microsatellite loci for genetic analysis of three populations of the tropical tree Eugenia uniflora L. (pitanga or Brazilian cherry) from fragments of semideciduous forest were developed. We used the technique of building a (GA)(n) and (CA)(n) microsatellite-enriched library by capture with streptavidin-coated magnetic beads. We assessed the polymorphism of seven microsatellites in 84 mature trees found in three areas (Ribeir (a) over tildeo Preto, Tambau and S (a) over tildeo Jose do Rio Pardo), highly impacted by the agricultural practices, in a large region among Pardo river and Mogi-Guacu river basins, in state of S (a) over tildeo Paulo, Brazil. All loci were polymorphic, and the number of alleles was high, ranging from 6 to 24, with a mean of 14.4. All stands showed the same high level of genetic diversity (mean H(E) = 0.83) and a low genetic differentiation (mean F(ST) = 0.031), indicating that genetic diversity was higher within rather than among populations. Seven of the nine loci were highly variable, and sufficiently informative for E. uniflora. It was concluded that these new SSR markers can be efficiently used for gene flow studies. (AU)

• Processo FAPESP: 03/04199-4 - Uso de marcadores SSR na análise genética de árvores matrizes vs. populações derivadas que formam parte do Banco de Germoplasma da Floresta da USP-RP
• Beneficiário: Ana Lilia Alzate Marin
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores